Preparation of vaccines using photosensitizer and light

• US 8,759,092 B2
• Filed: 08/26/2010
• Issued: 06/24/2014
• Est. Priority Date: 07/21/1998
• Status: Expired due to Fees

• Current Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Original Assignee: Terumo BCT Biotechnologies, LLC (Terumo Corporation)
• Inventors: Goodrich, Raymond P.
• Primary Examiner(s): CHEN, STACY BROWN
• Application Number:
  US12/869,526
• Publication Number:
  US 20110014239A1
• Time in Patent Office: 1,398 Days
• Field of Search: None
• US Class Current: 435/325
• CPC Class Codes: A61K 2039/5152 Tumor cells
• CPC Class Codes: A61K 2039/521 inactivated (killed)
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/0011 Cancer antigens
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/21 Retroviridae, e.g. equine infectious anemia virus
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C12N 2740/16034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2740/16063 by chemical treatment
• CPC Class Codes: C12N 2796/00 Viruses not covered by groups C12N2710/00 - C12N2795/00
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

Methods are provided for treating a vaccine containing infectious particles which may be viral, bacterial, and/or cellular in nature. Preferred methods include the steps of adding an effective, non-toxic amount of an endogenous photosensitizer to the fluid and exposing the fluid to photoradiation sufficient to inactivate the infectious particles but not enough to damage the antigenic characteristics of the infectious particles.

Claims

1. A method of making a composition comprising inactivated tumor cells, wherein the composition comprising the inactivated tumor cells is administered to a patient, the method comprising:

removing a tumor from the patient;

purifying at least some tumor cells from the tumor;

containing the tumor cells in a photopermeable container for radiation;

adding an endogenous photosensitizer to the photopermeable container, wherein the endogenous photosensitizer comprises 7,8-dimethyl-10-ribityl isoalloxazine, and wherein the endogenous photosensitizer is at a concentration of at least about 10 μ

M;

inactivating the tumor cells by exposing the tumor cells to the endogenous photosensitizer and light at a sufficient wavelength to prevent replication of the tumor cells but not substantially destroy the antigenic determinants of the tumor cells, wherein the exposing comprises;

irradiating the tumor cells and the endogenous photosensitizer in the photopermeable container at a range of between about 1 to about 120 J/cm² for a period of between about 6 to about 36 minutes;

suspending the inactivated tumor cells in a suspension solution to make the composition comprising the inactivated tumor cells, wherein the suspension solution comprises one or more from the group consisting of;

saline and water; and

administering the composition comprising the inactivated tumor cells to the patient subcutaneously, intramuscularly, intraperitoneally, orally, or nasally.

2. The method of claim 1, wherein the suspension solution comprises one or more from the group consisting of:

an antibiotic and a preservative.

3. The method of claim 1, wherein the suspension solution comprises an adjuvant.