Method for reducing DNA impurities in viral compositions
First Claim
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1. A method for the removal of DNA impurities from viral compositions, the method comprising the steps of:
- a) harvesting the virus from the cell culture supernatant without the use of a nuclease, followed by b) combining the virus with solution that has an ionic strength of between 0.5 mol/L and 3.0 mol/L followed by c) size exclusion chromatography.
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Abstract
A method for purification of viral compositions is provided. In particular, a method for reduction of unwanted residual DNA in a viral composition while retaining the immunogenicity of the virus itself is provided. The resulting immunogenic viral composition is substantially free of residual DNA, and is useful for the manufacture of medical products, such as vaccines designed for human or animal.
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7 Claims
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1. A method for the removal of DNA impurities from viral compositions, the method comprising the steps of:
- a) harvesting the virus from the cell culture supernatant without the use of a nuclease, followed by b) combining the virus with solution that has an ionic strength of between 0.5 mol/L and 3.0 mol/L followed by c) size exclusion chromatography.
- View Dependent Claims (2, 3, 4, 5, 6, 7)
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