Fragmented hydrogels

• US 8,795,727 B2
• Filed: 11/05/2010
• Issued: 08/05/2014
• Est. Priority Date: 11/09/2009
• Status: Active Grant

• Current Assignee: Spotlight Technology Partners LLC
• Original Assignee: Spotlight Technology Partners LLC
• Inventors: Gong, Glen, Pai, Suresh Subraya, Sershen, Scott Robert
• Primary Examiner(s): DICKINSON, PAUL W
• Application Number:
  US13/505,684
• Publication Number:
  US 20120238644A1
• Time in Patent Office: 1,369 Days
• Field of Search: None
• US Class Current: 424/484
• CPC Class Codes: A61K 31/225 Polycarboxylic acids
• CPC Class Codes: A61K 31/573 substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone
• CPC Class Codes: A61K 31/58 containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin digitoxin A61K31/7048
• CPC Class Codes: A61K 31/7036 having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins
• CPC Class Codes: A61K 31/722 Chitin, chitosan
• CPC Class Codes: A61K 47/10 Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
• CPC Class Codes: A61K 47/34 Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers A61K47/10 takes precedence
• CPC Class Codes: A61K 47/36 Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• CPC Class Codes: A61K 9/06 Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels composition of ointments, creams or gels A61K47/00
• CPC Class Codes: A61K 9/146 with organic macromolecular compounds
• CPC Class Codes: A61L 24/0031 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 24/08 Polysaccharides A61L24/043 takes precedence
• CPC Class Codes: A61L 26/0023 Polysaccharides
• CPC Class Codes: A61L 26/008 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/20 Polysaccharides
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 31/042 Polysaccharides
• CPC Class Codes: A61L 31/145 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: C08J 2371/02 : Polyalkylene oxides
• CPC Class Codes: C08J 2405/08 : Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: C08J 3/075 : Macromolecular gels
• CPC Class Codes: C08J 3/12 : Powdering or granulating
• CPC Class Codes: C08K 5/06 : Ethers; Acetals; Ketals; Ortho-esters
• CPC Class Codes: C09D 105/08 : Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof

Abstract

Fragmented polysaccharide based hydrogel compositions and methods of making and using the same are provided. The subject polysaccharide based hydrogel compositions are prepared by combining a polysaccharide component with a hydrophilic polymer and a cross-linking agent. Also provided are kits and systems for use in preparing the subject compositions.

Claims

1. A method of making a fragmented hydrogel composition, comprising:

combining in an alkaline solution of at least 30 mM chitosan, carboxymethylcellulose, carboxymethylchitosan, or a derivative thereof with a molecular weight of less than or equal to 5000 Dalton, a degree of deacetylation of 30% to 100%, a degree of deacetylation greater than or equal to 70%, and a multi-armed polyethylene glycol with a molecular weight of 10,000 Dalton to 30,000 Dalton and at least two nucleophilic groups;

combining a multi-armed polyethylene glycol with a molecular weight of 10,000 Dalton to 30,000 Dalton and at least two eletrophilic groups with an aqueous solution;

combining the alkaline and aqueous solution;

allowing the hydrogel composition to substantially cure;

drying the substantially cured hydrogel composition under elevated temperature and reduced pressure; and

fragmenting the dry hydrogel composition by cryogenic grinding or cryogenic milling.

2. A method of making a fragmented hydrogel composition, comprising:

combining in an alkaline solution of at least 30 mM chitosan, carboxymethylcellulose, carboxymethylchitosan, or a derivative thereof with a molecular weight of less than or equal to 5,000 Dalton, a degree of deacetylation of 30% to 100%, a degree of deacetylation greater than or equal to 70%, and a multi-armed polyethylene glycol with a molecular weight of 10,000 Dalton to 30,000 Dalton and at least two nucleophilic groups;

combining a multi-armed polyethylene glycol with a molecular weight of 10,000 Dalton to 30,000 Dalton and at least two eletrophilic groups with an aqueous solution;

combining the alkaline and aqueous solution;

allowing the hydrogel composition to substantially cure;

drying the substantially cured hydrogel composition under elevated temperature and reduced pressure;

fragmenting the dry hydrogel composition into a collection of particles by cryogenic grinding or cryogenic milling;

selecting a specific range of fragmenting hydrogel particulate; and

mixing the selected fragmented hydrogel particulate with a liquid.