Method for inducing migration of adipose-derived adult stem cells

• US 8,808,682 B2
• Filed: 10/25/2010
• Issued: 08/19/2014
• Est. Priority Date: 10/23/2009
• Status: Expired due to Fees

• Current Assignee: RNL Bio Co., Ltd.
• Original Assignee: RNL Bio Co., Ltd.
• Inventors: Ra, Jeong Chan, Kang, Sung Keun, Baek, Sun Jin
• Primary Examiner(s): Gamett, Daniel C
• Application Number:
  US13/502,898
• Publication Number:
  US 20120276044A1
• Time in Patent Office: 1,394 Days
• US Class Current: 424/93.1
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 2501/21 Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

The present invention relates to the ability of adipose tissue-derived adult stem cells to migrate, and more particularly to the novel use of adipose tissue-derived adult stem cells primed with a specific chemokine or growth factor and their specific secretory products, which more effectively migrate to a disease site in vivo. The inventive composition containing either adipose-derived adult stem cells or adipose tissue-derived adult stem cells primed with a specific chemokine or growth factor can be administered by a simple method such as intravenous administration and are able to induce the targeting of the stem cells to a disease site. Thus, the composition is useful as a cell therapeutic agent.

Claims

1. A method for inducing the migration of adipose tissue-derived adult stem cells, the method comprising the steps of:

priming adipose tissue-derived adult stem cells by culturing the cells in medium with added adiponectin; and

one or more factors selected from the group consisting of

TNF-α

(tumor necrosis factor-α

), PDGF-AB (platelet derived growth factor AB)

Rantes, SDF-1α

(stromal cell-derived factor-1α

), and MCP-1 (monocyte chemoattractant protein-1); and

administering a composition containing the primed adipose tissue-derived adult stem cells into an in vivo site which is not in direct contact with a disease site.

2. The method of claim 1, wherein the step of administering is performed by intravenous administration.

3. The method of claim 1, wherein the cells were primed for 24 hours.