Nucleic acid sequences encoding transcription factors regulating nicotine alkaloid biosynthesis and their use in modifying plant metabolism
First Claim
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1. An expression vector comprising a nucleic acid molecule comprising a nucleotide sequence wherein the nucleotide sequence is selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO;
14 or SEQ ID NO;
15;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
16; and
(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and encodes a transcription factor that negatively regulates nicotine biosynthesis;
wherein the nucleic acid molecule is operably linked to one or more heterologous control sequences suitable for directing expression in a Nicotiana cell.
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Abstract
Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
25 Citations
8 Claims
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1. An expression vector comprising a nucleic acid molecule comprising a nucleotide sequence wherein the nucleotide sequence is selected from the group consisting of:
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(a) the nucleotide sequence set forth in SEQ ID NO;
14 or SEQ ID NO;
15;(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
16; and(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and encodes a transcription factor that negatively regulates nicotine biosynthesis; wherein the nucleic acid molecule is operably linked to one or more heterologous control sequences suitable for directing expression in a Nicotiana cell. - View Dependent Claims (2, 3, 4)
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5. A nucleic acid molecule comprising a nucleotide sequence wherein the nucleotide sequence is selected from the group consisting of:
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(a) the nucleotide sequence set forth in SEQ ID NO;
14 or SEQ ID NO;
15;(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
16; and(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and encodes a transcription factor that negatively regulates nicotine biosynthesis; wherein the nucleic acid molecule is operably linked to one or more heterologous control sequences. - View Dependent Claims (6, 7, 8)
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Specification