Device for moving and treating volumes of liquid

• US 8,864,967 B2
• Filed: 06/30/2005
• Issued: 10/21/2014
• Est. Priority Date: 07/01/2004
• Status: Expired due to Fees

• Current Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Original Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Inventors: Marchand, Gilles, Fouillet, Yves, Clementz, Philippe
• Primary Examiner(s): LEONG, SUSAN DANG
• Application Number:
  US11/631,389
• Publication Number:
  US 20080302431A1
• Time in Patent Office: 3,400 Days
• Field of Search: 204400-435, 137 1- 9, 137825-826, 137/545, 4351734-1736, 435/446, 435/450, 422502-504, 205775-7945
• US Class Current: 204/409
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2300/1816 using induction heating
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: Y10T 137/206 Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system]
• CPC Class Codes: Y10T 137/2185 To vary frequency of pulses or oscillations

Abstract

A device for displacing a small volume of liquid under the effect of an electric control, including a first substrate with a hydrophobic surface provided with a first electrical conductor, a second electrical conductor positioned facing the first conductor, and a third conductor, forming with the second conductor, a mechanism for analyzing or heating a volume of liquid.

Claims

1. A device for displacing a volume of liquid under effect of an electric control, comprising:

a first substrate with a hydrophobic surface including first electrical conductors, the first electrical conductors comprising multiple individual first electrical conductors;

a second electrical conductor positioned facing each of the first individual electrical conductors;

a third electrical conductor positioned facing each of the first individual electrical conductors wherein the second and third electrical conductors are substantially parallel to the hydrophobic surface; and

a controller programmed to control a voltage applied to the first electrical conductors, the second electrical conductor, and the third electrical conductor, wherein

the controller is programmed to create a first potential difference between the first electrical conductors and the second electrical conductor to cause displacement of the volume of liquid, and

the controller is programmed to cause a second potential difference between the second electrical conductor and the third electrical conductor that induces a reaction in the volume of liquid or analyzes the volume of liquid when the second electrical conductor and the third electrical conductor both receive voltage and current flows through the volume of liquid from or to the second electrical conductor and the third electrical conductor such that the controller is programmed to use the second electrode for both said displacement of the volume of liquid and also for said inducing an electrochemical reaction in the volume of liquid or said analyzing the volume of liquid.

2. The device according to claim 1, wherein the second electrical conductor includes a catenary or a wire, substantially parallel to the hydrophobic surface.

3. The device according to claim 2, wherein the catenary or the wire is non-buried in the first substrate, at a non-zero distance from the hydrophobic surface.

4. The device according to claim 3, wherein the non-zero distance is between 1 μ

m and 500 μ

m.

5. The device according to claim 2, wherein the third electrical conductor includes a catenary or a conducting wire.

6. The device according to claim 5, wherein the catenary or wire of the third electrical conductor is non-buried in the first substrate, at a non-zero distance from the hydrophobic surface.

7. The device according to claim 6, wherein the non-zero distance is between 1 μ

m and 500 μ

m.

8. The device according to claim 5, wherein both catenaries or wires of the second and third electrical conductors are parallel to each other and to the hydrophobic surface, and both catenaries or wires of the second and third electrical conductors face the first electrical conductors.

9. The device according to claim 5, wherein both catenaries or wires of the second and third electrical conductors are not parallel to each other, but are parallel to the hydrophobic surface.

10. The device according to claim 2, wherein the third electrical conductor includes a catenary or a wire directed perpendicularly to the catenary or wire of the second electrical conductor.

11. The device according to claim 1, further including a second substrate with a hydrophobic surface, the second substrate giving a confined structure to the device.

12. The device according to claim 1, wherein the third conductor is a catenary.

13. The device according to claim 11, wherein the hydrophobic surface of the second substrate is locally apertured to form a contact area between a drop of liquid positioned between both the first and second substrates and the third electrical conductor.

14. The device according to claim 11, wherein the second substrate is positioned at a distance from the first substrate, between 10 μ

m and 500 μ

m.

15. The device according to claim 1, wherein the second and third electrical conductors are a catenary or wire.

16. The device according to claim 11, wherein at least one of the hydrophobic surface of the first substrate or of the second substrate includes polytetrafluoroethylene.

17. A method for treating a cell by electroporation comprising:

putting a cell into contact with the device according to claim 1; and

applying a potential difference between the first electrical conductors and the second electrical conductor.

18. A device for calibrating a drop of liquid comprising:

the device according to claim 1; and

means for controlling a current flowing between the second and the third electrical conductors.

19. The device according to claim 18, wherein the second and the third electrical conductors each include a catenary, both catenaries being positioned at different heights relative to the hydrophobic surface.

20. The device according to claim 19, further including at least an additional catenary, positioned at a distance from the hydrophobic surface, different from a distance between the hydrophobic surface and the catenaries of the second and third electrical conductors.

21. The device according to claim 5, wherein both catenaries or wires of the second and third electrical conductors are disposed along non-intersecting directions and face the first electrical conductors.

22. The device according to claim 1, wherein both of the second and third electrical conductors are substantially parallel to the hydrophobic surface, both of the second and third electrical conductors facing a same first electrical conductor.

23. The device according to claim 1, wherein both of the second and third electrical conductors are substantially parallel to the hydrophobic surface, both of the second and third electrical conductors being disposed directly over a same one of said first electrical conductors.

25. The device of claim 1, wherein the controller is programmed to use the second and third electrodes to electrically induce a reaction in the volume of liquid and to electrically analyze the volume of liquid.

24. A device for displacing a volume of liquid under effect of an electric control, comprising:

a first substrate with a hydrophobic surface including first electrical conductors, the first electrical conductors comprising multiple individual first electrical conductors;

a second electrical conductor positioned facing each of the first individual electrical conductors; and

a third electrical conductor positioned facing each of the first individual electrical conductors, wherein the second and third electrical conductors are substantially parallel to the hydrophobic surface, and wherein

a first potential difference between the first electrical conductors and the second electrical conductor causes displacement of the volume of liquid, and

both of the second and third electrical conductors are substantially parallel to the hydrophobic surface and to each other, both of the second and third electrical conductors being disposed directly over a same one of said first electrical conductors, such that the second electrical conductor is a dual function electrode for both displacement of the volume of liquid and also analysis of the volume of liquid.

26. The device of claim 24, wherein the second and third electrical conductors electrically induce a reaction in the volume of liquid and electrically analyze the volume of liquid.