Hydrodynamic focusing devices

• US 8,883,091 B2
• Filed: 06/09/2006
• Issued: 11/11/2014
• Est. Priority Date: 06/26/2003
• Status: Expired due to Fees

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Sundararajan, Narayanan, Berlin, Andrew
• Primary Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US11/450,083
• Publication Number:
  US 20070036678A1
• Time in Patent Office: 3,077 Days
• Field of Search: 422/100, 422/103, 422/82.05, 137/89, 137/110
• US Class Current: 422/502
• CPC Class Codes: B01F 33/3011 using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A micro-fluidic device containing a micro-fluidic inlet channel to convey a process flow, a plurality of micro-fluidic focusing channels to each convey one of a plurality of focusing flows, a focusing manifold coupled with the inlet channel at an inlet port thereof and with the plurality of focusing channels at a plurality of focusing channel ports thereof to focus the process flow by contacting and hydrodynamically impacting at least three sides of the process flow with the focusing flows, and a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the combined focused process flow and focusing flow from the focusing manifold.

Claims

1. An analysis system comprising a hydrodynamic focusing device comprising:

a micro-fluidic inlet channel to convey a process flow;

at least four micro-fluidic focusing channels to each convey one of at least four focusing flows, the at least four micro-fluidic focusing channels comprising a first channel, a second channel, a third channel and a fourth channel;

a focusing manifold having a plurality of areas coupled with the micro-fluidic inlet channel at an inlet port and with the at least four micro-fluidic focusing channels at a plurality of focusing channel ports, wherein the focusing manifold is configured to focus the process flow by contacting the process flow with the at least four focusing flows from at least four different directions to form a focused process flow, wherein each different direction is from a different area of the focusing manifold; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the focused process flow and the focusing flows from the focusing manifold; and

an analysis device to analyze the focused process flow;

wherein the focused process flow is separated from each wall of the micro-fluidic outlet channel with the at least four focusing flows;

wherein the first and second channels are parallel and coplanar with the micro-fluidic inlet channel in a vertical plane;

wherein the third and fourth channels are angled with respect to and coplanar with the micro-fluidic inlet channel in a horizontal plane.

2. The analysis system of claim 1, wherein the analysis device being located at a location such that the interrogation signal or beam is configured to analyze the focused process flow.

3. The analysis system of claim 1, further comprising a source of a biological molecule coupled with the micro-fluidic inlet channel.

4. The analysis system of claim 3, wherein the source of the biological molecule comprises a source of a fluorescently labeled biological molecule.

5. The analysis system of claim 3, wherein the source of the biological molecule comprises a source of a nucleic acid derivative.

6. The analysis system of claim 3, wherein the source of the biological molecule comprises a source of a protein.

7. The analysis system of claim 1, wherein the focused process flow has a cross-sectional dimension in a range of 0.1 μ

m to 50 μ

m.

8. The analysis system of claim 1, wherein the focused process flow has a cross-sectional dimension in a range of 0.1 μ

m to 10 μ

m.

9. The analysis system of claim 2, wherein the process flow a cross-sectional dimension in a range of 0.1 μ

m to 50 μ

m.

10. The analysis system of claim 2, wherein the process flow has a cross-sectional dimension in a range of 0.1 μ

m to 10 μ

m.

11. The analysis system of claim 1, wherein the analysis device comprises a Raman spectrometer.

12. The analysis system of claim 1, wherein the analysis system is configured to focus the process flow by surrounding and spatially confining the process flow with the at least four focusing flows.

13. The analysis system of claim 2, wherein the analysis system is configured to focus the process flow by contacting the process flow from at least four different directions with the at least four focusing flows.

18. The analysis system of claim 1, wherein the at least four micro-fluidic focusing channels are not coplanar.

20. The analysis system of claim 1, wherein at least one of the at least four micro-fluidic focusing channels is not substantially parallel with another of the at least four micro-fluidic focusing channels.

14. An analysis system comprising a hydrodynamic focusing device comprising:

a micro-fluidic inlet channel to convey a process flow;

at least four micro-fluidic focusing channels to each convey one of at least four of focusing flows, the at least four micro-fluidic focusing channels comprising a first channel, a second channel, a third channel and a fourth channel;

a focusing manifold coupled with the micro-fluidic inlet channel at an inlet port and with the at least four micro-fluidic focusing channels at least four focusing channel ports, wherein the focusing manifold is configured to focus the process flow by contacting the process flow with the at least four focusing flows to form a focused process flow having a first cross sectional area, wherein each of the focusing flows is from a different area of the focusing manifold; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the focused process flow and the focusing flows from the focusing manifold, the micro-fluidic outlet channel comprising a polygonal cross-section with a least four sides;

wherein the focused process flow is separated from each wall of the micro-fluidic outlet channel with the at least four focusing flows;

wherein the first and second channels are parallel and coplanar with the micro-fluidic inlet channel in a vertical plane;

wherein the third and fourth channels are angled with respect to and coplanar with the micro-fluidic inlet channel in a horizontal plane.

15. The analysis system of claim 14, further comprising an analysis device configured to emit an interrogation signal or beam having a second cross sectional area to analyze the focused process flow having the first cross sectional area, wherein the first cross sectional area is smaller than the second cross sectional area, the analysis device being located at a location such that the interrogation signal or beam is configured to analyze the focused process flow.

16. The analysis system of claim 14, wherein the process flow is separated from a wall of the micro-fluidic outlet channel with the at least four focusing flows.

17. The analysis system of claim 14, wherein the focused process flow has a cross sectional dimension that is not greater than a cross sectional dimension of an interrogation signal associated with an analysis of the focused process flow.

19. The analysis system of claim 14, wherein the at least four micro-fluidic focusing channels are not coplanar.

21. The analysis system of claim 14, wherein at least one of the at least four micro-fluidic focusing channels is not substantially parallel with another of the at least four micro-fluidic focusing channels.