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Systems and methods of fluidic sample processing

  • US 8,883,518 B2
  • Filed: 03/30/2012
  • Issued: 11/11/2014
  • Est. Priority Date: 08/06/2007
  • Status: Active Grant
First Claim
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1. A method of detecting an analyte in a bodily fluid from a subject, comprising:

  • a) providing a fluidic device comprising a sample collection unit, an assay assembly, and a calibration unit, wherein,i) said sample collection unit is configured to collect a sample of the bodily fluid from said subject, comprises an anticoagulant, and is removable from the fluidic device;

    ii) said assay assembly comprises at least one reaction site containing a reactant configured to react with said analyte to yield a detectable signal indicative of the presence of said analyte; and

    iii) said calibration unit is configured to provide a measurement of said sample used for calibrating said detectable signal;

    b) allowing a portion of said sample to react with assay reagents contained within said assay assembly to yield a signal indicative of the presence of said analyte in said sample; and

    c) detecting said signal generated from said analyte in said sample of bodily fluid.

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