Fed-batch method of making human anti-TNF-alpha antibody

  • US 8,906,646 B2
  • Filed: 03/26/2014
  • Issued: 12/09/2014
  • Est. Priority Date: 09/13/2006
  • Status: Active Grant
First Claim
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1. A fed-batch method of making a human anti-TNFα

  • antibody comprising a light chain variable region (LCVR) comprising the sequence of SEQ ID NO;

    1 and a heavy chain variable region (HCVR) comprising the sequence of SEQ ID NO;

    2, said method comprising culturing Chinese Hamster Ovary (CHO) cells comprising a nucleic acid encoding said LCVR and HCVR of said anti-TNFα

    antibody in a cell culture production medium in large-scale,wherein glucose concentration in said cell production medium is monitored, the glucose concentration in said medium decreases to below 2 g/L, and glucose is added to said medium when the glucose concentration in said medium decreases to below 2 g/L, or the glucose concentration in said medium is monitored and glucose is added to said medium to maintain the glucose concentration in said medium at a concentration of at least 2 g/L but no greater than 7 g/L, such that said anti-TNFα

    antibody is produced, and wherein said produced antibody is further affinity purified using a Protein A resin.

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