Generation and maintenance of stem cells
First Claim
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1. A method of culturing pluripotent cells through at least one cell division, the method comprising, culturing pluripotent animal cells in the presence of a sufficient amount of:
- a) an ALK5 inhibitor;
b) a second compound selected from one or more of a MEK inhibitor, an Erk inhibitor, a p38 inhibitor, and an FGF receptor inhibitor;
c) a GSK3β
inhibitor; and
d) nutrients;
to allow for at least one cell division while maintaining cell pluripotency.
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Abstract
The present invention provides for the generation and maintenance of pluripotent cells by culturing the cells in the presence of an ALK5 inhibitor.
61 Citations
20 Claims
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1. A method of culturing pluripotent cells through at least one cell division, the method comprising, culturing pluripotent animal cells in the presence of a sufficient amount of:
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a) an ALK5 inhibitor; b) a second compound selected from one or more of a MEK inhibitor, an Erk inhibitor, a p38 inhibitor, and an FGF receptor inhibitor; c) a GSK3β
inhibitor; andd) nutrients; to allow for at least one cell division while maintaining cell pluripotency. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A culture of pluripotent mammalian cells, comprising a sufficient amount of:
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a) an ALK5 inhibitor; b) a second compound selected from one or more of a MEK inhibitor and an FGF receptor inhibitor; and c) a GSK3β
inhibitor;to allow for at least one cell division while maintaining cell pluripotency. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A cell culture medium, comprising a sufficient amount of:
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a) an ALK5 inhibitor; b) a second compound selected from one or more of a MEK inhibitor and an FGF receptor inhibitor; and c) a GSK3β
inhibitor;to allow for at least one cell division while maintaining cell pluripotency when pluripotent cells are cultured in the medium. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification