Fed-batch method of making human anti-TNF-alpha antibody

  • US 8,911,964 B2
  • Filed: 03/26/2014
  • Issued: 12/16/2014
  • Est. Priority Date: 09/13/2006
  • Status: Active Grant
First Claim
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1. A fed-batch production method of making a human anti-TNFα

  • antibody which comprises (1) a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO;

    1 and (2) a heavy chain variable region comprising the amino acid sequence of SEQ ID NO;

    2, said method comprising culturing Chinese Hamster Ovary (CHO) cells comprising a nucleic acid encoding said anti-TNFα

    antibody in a cell culture production medium in large-scale, wherein the glucose concentration in said medium is monitored, the glucose concentration in said medium decreases to below 2 g/L, and glucose is added to said medium when the glucose concentration in said medium decreases to below 2 g/L, or the glucose concentration in said medium is monitored and glucose is added to said medium to maintain the glucose concentration in said medium at a concentration of at least 2 g/L but no greater than 7 g/L, such that said anti-TNFα

    antibody is produced at a titer of at least 2 g/L in said cell culture production medium.

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