Methods of use for surface enhanced spectroscopy-active composite nanoparticles

• US 8,918,161 B2
• Filed: 10/03/2008
• Issued: 12/23/2014
• Est. Priority Date: 10/06/1999
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Natan, Michael J., Doering, William E.
• Primary Examiner(s): Kish, James
• Assistant Examiner(s): FISHER, MICHAEL NAPOLEON
• Application Number:
  US12/245,538
• Publication Number:
  US 20110172523A1
• Time in Patent Office: 2,272 Days
• Field of Search: 600/310, 600/324, 600/329, 600/332, 600/407, 600/410, 600475-476, 356/301, 424/178, 424/179, 436546-547, 530/391
• US Class Current: 600/431
• CPC Class Codes: B22F 1/054 Nanosized particles
• CPC Class Codes: B22F 1/0547 Nanofibres or nanotubes
• CPC Class Codes: B22F 1/0553 Complex form nanoparticles, e.g. prism, pyramid, octahedron
• CPC Class Codes: B22F 1/056 Submicron particles having a size above 100 nm up to 300 nm
• CPC Class Codes: B22F 1/102 Metallic powder coated with organic material
• CPC Class Codes: B22F 1/17 Metallic particles coated with metal
• CPC Class Codes: B22F 2998/00 Supplementary information concerning processes or compositions relating to powder metallurgy
• CPC Class Codes: B22F 9/14 using electric discharge
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C25D 1/006 Nanostructures, e.g. using aluminium anodic oxidation templates [AAO]
• CPC Class Codes: C25D 1/04 Wires; Strips; Foils
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 33/54346 Nanoparticles
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

Various methods of use for surface enhanced spectroscopy-active composite nanoparticles (SACN'"'"'s) are provided. SACN'"'"'s are submicron-sized particles or labels can be covalently or non-covalently affixed to entities of interest for the purpose of quantification, location, identification, tracking, and diagnosis. The various methods include administering a SACN nanoparticle imaging agent to a patient, scanning the patient using a system that can perform spectral imaging; and generating a spectrum or image of an internal region of the patient. A method, for diagnosing an abnormal pathology as well as a method for labeling an animal with a SACN are also provided.

Claims

1. A method comprising,

a) administering a Surface Enhanced Spectroscopy-Active Nanoparticle (SACN) imaging agent to a patient,

b) performing a spectral imaging of the patient;

and

c) generating a spectrum or image of an internal region of the patient by detecting said SACN,

wherein said SACN comprises a metal nanoparticle core, a raman-active reporter, and an encapsulant.

2. The method of claim 1, wherein said spectral imaging is conducted by a spot scanning confocal microscope, a line scanning system, an Optical Coherence tomographic system, a system that detects only over a single wavelength band, a fluorescence imaging system comprising an excitation light source or filtered image detection.

3. The method of claim 1, wherein said internal region of a patient is selected from the group consisting of the entire internal region of a patient, the vasculature, heart, liver, and spleen, the gastrointestinal region.

4. The method of claim 1, wherein the system that can perform spectral imaging is a portable system.

5. The method of claim 1, wherein said SACN is non-covalently associated with an imaging probe, wherein said administering comprises introducing the imaging probe into said internal region of said patient, and wherein said SACNs disassociate into the tissue of said patient or a bodily fluid of said patient.

6. A method, comprising:

a) introducing a plurality of Surface Enhanced Spectroscopy-Active Composite Nanoparticles (SACNs) targeted to a molecule involved in an abnormal pathology into a patient with the abnormal pathology, wherein the SACNs become associated to said molecule involved with the abnormal pathology; and

b) obtaining an imaging of the associated SACNs, whereby an abnormal pathology may be diagnosed,

wherein each SACN of said plurality comprises a metal nanoparticle core, a ramam-active reporter, and an encapsulant.

7. The method of claim 6, wherein said the location of the abnormal pathology is selected from the group consisting of the gastro-intestinal tract, heart, lung, liver cervix, breast, and colon.

8. A method for labeling an animal with a Surface Enhanced Spectroscopy-Active Composite Nanoparticle (SACN), comprising the steps of:

introducing a SACN into an animal;

detecting the SACN or labeled animal, wherein said introducing is selected from the group consisting of subcutaneous implantation and intravenous introduction; and

wherein said SACN comprises a metal nanoparticle core, a raman-active reporter, and an encapsulant,

wherein said introducing is selected from the group consisting of subcutaneous implantation and intravenous introduction,

wherein said SACN comprises a metal nanoparticle core, a raman-active reporter, and an encapsulant.