Scaffolds for cell transplantation

• US 8,932,583 B2
• Filed: 11/28/2011
• Issued: 01/13/2015
• Est. Priority Date: 12/13/2005
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation), Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation), Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Mooney, David J., Barros e Silva, Eduardo Alexandre, Hill, Elliot Earl Jr.
• Primary Examiner(s): Long, Scott
• Application Number:
  US13/305,088
• Publication Number:
  US 20120134967A1
• Time in Patent Office: 1,142 Days
• Field of Search: None
• US Class Current: 424/93.7
• CPC Class Codes: A61K 2039/54 characterised by the route of administration
• CPC Class Codes: A61K 2039/55516 Proteins; Peptides
• CPC Class Codes: A61K 2039/55522 Cytokines; Lymphokines; Interferons
• CPC Class Codes: A61K 2039/55561 CpG containing adjuvants; Oligonucleotide containing adjuvants
• CPC Class Codes: A61K 2039/55583 Polysaccharides
• CPC Class Codes: A61K 2039/6093 Synthetic polymers, e.g. polyethyleneglycol [PEG], Polymers or copolymers of (D) glutamate and (D) lysine
• CPC Class Codes: A61K 38/1866 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: A61K 38/193 Colony stimulating factors [CSF]
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 39/0011 Cancer antigens
• CPC Class Codes: A61K 39/001156 Tyrosinase and tyrosinase related proteinases [TRP-1 or TRP-2]
• CPC Class Codes: A61K 39/00117 Mucins, e.g. MUC-1
• CPC Class Codes: A61K 39/001171 Gangliosides, e.g. GM2, GD2 or GD3
• CPC Class Codes: A61K 39/00118 from embryonic or fetal origin
• CPC Class Codes: A61K 39/001182 Carcinoembryonic antigen [CEA]
• CPC Class Codes: A61K 39/001184 Cancer testis antigens, e.g. SSX, BAGE, GAGE or SAGE
• CPC Class Codes: A61K 39/001186 MAGE
• CPC Class Codes: A61K 39/001191 Melan-A/MART
• CPC Class Codes: A61K 39/001192 Glycoprotein 100 [Gp100]
• CPC Class Codes: A61K 39/001195 Prostate specific membrane antigen [PSMA]
• CPC Class Codes: A61K 39/21 : Retroviridae, e.g. equine infectious anemia virus
• CPC Class Codes: A61K 39/39 : characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61K 9/0024 : Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61K 9/1647 : Polyesters, e.g. poly(lactide-co-glycolide)
• CPC Class Codes: A61L 2300/232 : Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
• CPC Class Codes: A61L 2300/252 : Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/412 : Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2300/414 : Growth factors
• CPC Class Codes: A61L 2300/426 : Immunomodulating agents, i.e. cytokines, interleukins, interferons
• CPC Class Codes: A61L 2300/438 : Antigens
• CPC Class Codes: A61L 2300/604 : Biodegradation
• CPC Class Codes: A61L 2300/606 : Coatings
• CPC Class Codes: A61L 2400/18 : Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 2430/30 : for muscle reconstruction
• CPC Class Codes: A61L 2430/34 : for soft tissue reconstruction
• CPC Class Codes: A61L 27/18 : obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/20 : Polysaccharides
• CPC Class Codes: A61L 27/3633 : Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/38 : containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3826 : Muscle cells, e.g. smooth muscle cells
• CPC Class Codes: A61L 27/54 : Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 : Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 : Materials at least partially resorbable by the body
• CPC Class Codes: A61P 35/00 : Antineoplastic agents
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: B33Y 80/00 : Products made by additive manufacturing
• CPC Class Codes: C12N 15/117 : Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
• CPC Class Codes: C12N 2310/17 : Immunomodulatory nucleic acids
• CPC Class Codes: C12N 2320/32 : Special delivery means, e.g. tissue-specific
• CPC Class Codes: C12N 2740/16034 : Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 : Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

A device that includes a scaffold composition and a bioactive composition with the bioactive composition being incorporated into or coated onto the scaffold composition such that the scaffold composition and/or a bioactive composition controls egress of a resident cell or progeny thereof. The devices mediate active recruitment, modification, and release of host cells from the material.

Claims

1. A method of regenerating a target tissue of a mammal, comprising contacting a mammalian musculoskeletal tissue with a device comprising a scaffold composition with a bioactive composition being incorporated therein or thereon and a mammalian myogenic passenger cell bound to said scaffold,

wherein said bioactive composition comprises an outward migration-inducing factor,

wherein the scaffold composition comprises, open, interconnected macropores of 400-500 μ

m in size,

wherein said scaffold composition temporally controls egress of said passenger cell,

wherein release of the outward migration-inducing factor from the scaffold composition over time promotes migration of said passenger cell out of the scaffold composition,

wherein the outward migration-inducing factor comprises hepatocyte growth factor (HGF) and a member of the fibroblast growth factor (FGF) family that has been demonstrated to have a physiological role in skeletal muscle regeneration, and

wherein said passenger cell migrates out of the scaffold and grafts into said target tissue, thereby regenerating or repairing said target tissue.

2. The method of claim 1, wherein said myogenic cell comprises a myoblast, satellite cell, or muscle progenitor cell.

3. The method of claim 1, wherein said member of the fibroblast growth factor (FGF) family comprises fibroblast growth factor 2 (FGF2).

4. The method of claim 1, wherein said bioactive composition comprises insulin-like growth factor (IGF).

5. The method of claim 1, wherein said scaffold composition comprises an additional growth factor, cytokine, or small molecule mimic thereof.

6. The method of claim 1, wherein the scaffold composition comprises aligned macropores.

7. The method of claim 1, wherein the outward migration-inducing factor further comprises an arginine-glycine-aspartate (RGD)-containing peptide.