Electromagnetic field therapy delays cellular senescence and death by enhancement of the heat shock response

• US 9,014,801 B2
• Filed: 03/19/2009
• Issued: 04/21/2015
• Est. Priority Date: 03/19/2008
• Status: Active Grant

• Current Assignee: Board of Trustees of The University of Illinois
• Original Assignee: Board of Trustees of The University of Illinois
• Inventors: Perez, Felipe Pablo, Jurivich, Donald A., Morisaki, Jorge Javier, Zhou, Ximing
• Primary Examiner(s): DIETRICH, JOSEPH M
• Application Number:
  US12/407,616
• Publication Number:
  US 20090276019A1
• Time in Patent Office: 2,224 Days
• Field of Search: 607/2
• US Class Current: 607/2
• CPC Class Codes: A61N 1/403 for thermotherapy, e.g. hyperthermia
• CPC Class Codes: A61N 5/02 using microwaves
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Disclosed herein is a method comprising repetitive electromagnetic field shock to at least one living cell, wherein the repetitive electromagnetic field shock improves HSF1 and/or HSR function, and producing delaying and reversal of aging and age related diseases. Also disclosed herein is an apparatus adapted to deliver repetitive electromagnetic field shock to at least one living cell, wherein the repetitive electromagnetic field shock improves HSF1 and HSR function.

Claims

1. A method comprising:

applying repetitive radio frequency electromagnetic field shock to at least one living cell, wherein the repetitive radio frequency electromagnetic field shock is configured to precondition improvement in either heat shock factor 1 (HSF1) or heat shock response (HSR) function, or HSF1 and HSR function, and increasing expression of heat shock proteins (HSPs) without increasing temperature or causing external heat damage, wherein the precondition improvement is characterized in an increase in HSF1-heat shock element (HSE) binding.

2. The method of claim 1, wherein the applying of the repetitive radio frequency electromagnetic field shock is configured to disrupt the molecular brake of HSF1-DNA binding without resulting in a decline of Hsp90.

3. The method of claim 1, wherein the applying repetitive radio frequency electromagnetic field shock is at about 50 MHz/0.5 W continuous radiation.

4. The method of claim 1, wherein the applying repetitive radio frequency electromagnetic shock is between about 5 minutes to about 30 minutes in duration and is repeated for a period selected from the group consisting of:

once a day, about once every two days, about once every three days, about once a week, and about twice a week.

5. The method of claim 1, wherein the applying repetitive radio frequency electromagnetic field shock delays or reverses senescence.

6. The method of claim 5, wherein the at least one living cell is a plurality of T-cells and at least a portion of the applying repetitive radio frequency electromagnetic field shock is applied to the T-cells during IL-2 stimulation.

7. The method of claim 5, wherein at least a portion of the application of the repetitive radio frequency electromagnetic field shock is applied concurrently with a treatment that augments the DAF-16 (FOXO) pathway.

8. The method of claim 1, wherein the repetitive radio frequency electromagnetic field shock is applied to the at least one cell until the cell reaches cellular senescence to delay at least one aging characteristic.

9. The method of claim 8, wherein the at least one aging characteristic is a decline in cell proliferation rates or terminal lifespan or senescent phenotype.

10. The method of claim 1, wherein the at least one cell is a T cell.

11. The method of claim 1, wherein the at least one living cell is a plurality of living cells, and the method further comprises applying repetitive radio frequency electromagnetic field shock to a plurality of in vivo living cells.

12. The method of claim 11, wherein the in vivo living cells are brain cells.

13. The method of claim 1, wherein the repetitive radio frequency electromagnetic field shock is delivered to at least a localized region of the body of a patient.

14. The method of claim 1, wherein the repetitive radio frequency electromagnetic field shock is provided by a transversal electromagnetic chamber.

15. The method of claim 1, wherein the at least one living cell is a plurality of living cells and the applying repetitive radio frequency electromagnetic field shock extends the replicative lifespan of the at least one living cell.

16. The method of claim 15, wherein the applying repetitive radio frequency electromagnetic field shock extends the terminal lifespan of at least one living cell of the plurality of living cells.

17. The method of claim 1, wherein the at least one living cell is a plurality of living fibroblast cells and the applying repetitive radio frequency electromagnetic field shock delays the average age-related enlargement and diversification of at least one fibroblast cell.

18. The method of claim 1, wherein the at least one living cell is a plurality of living fibroblast cells and the applying repetitive radio frequency electromagnetic field shock reverts at least one fibroblast cell to a more youthful phenotype.

19. The method of claim 1, wherein the applying repetitive radio frequency electromagnetic field shock to the at least one living cell treats at least one age related disease.

20. The method of claim 19, wherein the at least one age related disease is Alzheimer'"'"'s disease.

21. A method comprising:

applying repetitive radio frequency electromagnetic field shock to at least one living cell, wherein the repetitive radio frequency electromagnetic field shock is configured to precondition enhancement of the heat shock response (HSR)/heat shock factor 1 (HSF1) pathway and augment chaperone induction during stress, and increasing expression of heat shock proteins (HSPs) without increasing temperature or causing external heat damage wherein the precondition enhancement is characterized in an increase of HSF1-heat shock element (HSE) binding.

22. The method of claim 21, wherein the applying of the repetitive radio frequency electromagnetic field shock is configured to disrupt the molecular brake of HSF1-DNA binding without resulting in a decline of Hsp90.

23. The method of claim 21, wherein the applying repetitive radio frequency electromagnetic field shock is at about 50 MHz/0.5 W continuous radiation.