Isolation and purification of antibodies using protein a affinity chromatography

  • US 9,018,361 B2
  • Filed: 03/26/2014
  • Issued: 04/28/2015
  • Est. Priority Date: 10/20/2008
  • Status: Active Grant
First Claim
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1. A process for purifying adalimumab from a fermentation harvest of a Chinese Hamster Ovary (CHO) cell culture expressing said adalimumab, said process comprising:

  • a) binding adalimumab from said fermentation harvest to a Protein A resin,b) eluting the bound adalimumab at an elution pH of 3.6-4, andc) incubating the eluted adalimumab for 1 to 3 hours.

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