Use and making of biosensors utilizing antimicrobial peptides for highly sensitive biological monitoring

• US 9,029,168 B2
• Filed: 06/28/2011
• Issued: 05/12/2015
• Est. Priority Date: 06/28/2010
• Status: Active Grant

• Current Assignee: The Trustees of Princeton University (Princeton University)
• Original Assignee: The Trustees of Princeton University (Princeton University)
• Inventors: McAlpine, Michael C., Mannoor, Manu Sebastian
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US13/171,120
• Publication Number:
  US 20120156688A1
• Time in Patent Office: 1,414 Days
• Field of Search: 436/518
• US Class Current: 436/518
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 33/56916 Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

A biosensor and method of making are disclosed. The biosensor is configured to detect a target and may include a peptide immobilized on a sensing component, the sensing component having an anode and a cathode. The immobilized peptide may comprise an antimicrobial peptide binding motif for the target. The sensing component has an electrical conductivity that changes in response to binding of the immobilized peptide to the target. The immobilized peptide may bind one or more targets selected from the list consisting of: bacteria, Gram-negative bacteria, Gram-positive bacteria, pathogens, protozoa, fungi, viruses, and cancerous cells. The biosensor may have a display with a readout that is responsive to changes in electrical conductivity of the sensing component. The display unit may be wirelessly coupled to the sensing component. A resonant circuit with an inductive coil may be electrically coupled to the sensing component. A planar coil antenna may be disposed in proximity to the resonant circuit, the planar coil antenna being configured to provide power to the sensing component.

Claims

1. A biosensor configured for application to a biological surface, the biosensor comprising:

a microelectrode array disposed on a dissolvable substrate;

an anode and a cathode electrically coupled to the microelectrode array;

a resonant circuit with an inductive coil electrically coupled to the cathode and anode; and

a wireless telemetry unit electrically coupled to the resonant circuit, forming an electric circuit,

the dissolvable substrate being configured to be dissolved off by the application of water, leaving the microelectrode array, resonant circuit and wireless telemetry unit intimately attached to the biological surface.

2. The biosensor of claim 1, further comprising:

a binding molecule disposed on the microelectrode array, the microelectrode array having an electrical conductivity that is dependent on a binding state of the binding molecule.

3. The biosensor of claim 2, wherein binding molecule is selected from the list consisting of:

antimicrobial peptides, antibodies, modified antimicrobial peptides, modified antibodies, chimeric peptides containing antimicrobial peptide binding motifs, chimeric peptides containing antibody binding motifs, DNA fragments, RNA fragments, peptide binding motifs, proteins, small molecules and polymers combinations thereof.

4. The biosensor of claim 1, wherein the dissolvable substrate is selected from a list consisting of:

poly(ethylene terepthalate), poly(imide), poly(ether sulfone), cellulose, paper, silk, silk fibroin and combinations thereof.

5. The biosensor of claim 1, wherein the dissolvable substrate is placed in contact with a biologic surface.

6. The biosensor of claim 5, wherein the biologic surface is capable of bioresorption.

7. The biosensor of claim 5, wherein the biologic surface is selected from the list consisting of:

teeth, bone, skin, tissue, hair, nail, cornea, gum, tongue, palate, brain, heart, lung, membrane, leaf, root, bark, fur, feather, chiton and scale.

8. The biosensor of claim 1, further comprising:

a receiver unit having a planar coil antenna electrically coupled to a readout, the planar coil antenna being configured to power the electric circuit, enabling the wireless telemetry unit to send a wireless signal corresponding to an electrical conductivity of the circuit to the receiver unit, the readout being responsive to changes in the electrical conductivity of the circuit.

9. The biosensor of claim 1, wherein the binding molecule is contains a glucose-binding motif.

10. The biosensor of claim 1, wherein the microelectrode array is interdigitated.

11. The biosensor of claim 1, wherein the microelectrode array comprises graphene.