Signal activated RNA interference

• US 9,029,524 B2
• Filed: 12/10/2008
• Issued: 05/12/2015
• Est. Priority Date: 12/10/2007
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Han, Si-Ping, Barish, Robert D., Goddard, William A. III
• Primary Examiner(s): Angell, Jon E.
• Application Number:
  US12/316,372
• Publication Number:
  US 20090234109A1
• Time in Patent Office: 2,344 Days
• Field of Search: 536/24.5
• US Class Current: 536/24.5
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C12N 15/111 General methods applicable to biologically active non-coding nucleic acids
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/11 Antisense
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12N 2310/141 MicroRNAs, miRNAs
• CPC Class Codes: C12N 2310/3517 Marker; Tag
• CPC Class Codes: C12N 2310/3519 Fusion with another nucleic acid
• CPC Class Codes: C12N 2310/53 partially self-complementary or closed
• CPC Class Codes: C12N 2320/10 in screening processes

Abstract

The invention provides compositions and methods for signal activated RNA interference (saRNAi), preferably in vivo. The invention provides polynucleotides that switches between an inactive form and an active form upon covalent or non-covalent interaction with one or more specific chemical signals, such as disease-specific mRNA, miRNA, or other cellular RNA products with sequences that characterize diseased states of the cell. The interaction between the subject polynucleotides and the signals is preferably mediated by hybridization, which exposes, facilitates the formation, and/or allows the formation of a substrate that can be processed by proteins of the RNAi pathway (such as Dicer). The input and output of multiple different polynucleotides of the invention can form an in vivo signaling network. In addition, the multiple input signals can be integrated to modulate the activity of the subject polynucleotides.

Claims

1. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides are calculated to lack sequence identity with other sequences in the human genome.

2. The signal-activated polynucleotide construct of claim 1, wherein in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence to form a three-arm junction.

3. The signal-activated polynucleotide construct of claim 1, comprising two or more single stranded polynucleotides.

4. The signal-activated polynucleotide construct of claim 1, wherein in the absence of the signal polynucleotides, the guide sequence and the sense sequence form a short duplex region of no more than 19 base pairs in length.

5. The signal-activated polynucleotide construct of claim 4, wherein the short duplex region comprises one or more chemical modifications that confer resistance to nuclease degradation.

6. The signal-activated polynucleotide construct of claim 1, wherein in the absence of the signal polynucleotides, the guide sequence and the sense sequence form a double stranded region comprising one or more chemical modifications that inhibits Dicer cleavage and/or productive RISC incorporation.

7. The signal-activated polynucleotide construct of claim 1, wherein said part of the guide sequence and said part of the sense sequence, when forming part of said duplex region, comprises one or more mismatched base pairs.

10. The signal-activated polynucleotide construct of claim 1,

wherein the guide sequence comprises a 2-nucleotide 3′

overhang.

24. The signal-activated polynucleotide construct of claim 1, wherein the signal detection sequence comprise one or more deoxyribonucleotides or chemically modified ribonucleotides.

25. The signal-activated polynucleotide construct of claim 24, wherein said chemically modified ribonucleotides have one or more modifications on the ribose sugar ring, the phosphodiester backbone, and/or the base.

27. The signal-activated polynucleotide construct of claim 1, wherein said signal detecting sequence comprise one or more chemical modifications that promote hybridization between said signal detection sequence and said signal polynucleotides.

28. The signal-activated polynucleotide construct of claim 1, comprising one or more chemical modifications that facilitate the delivery of said construct.

29. The signal-activated polynucleotide construct of claim 28, wherein said chemical modifications enhance non-covalent association with one or more of:

cholesterol, antibody, nanoparticle, polymer, dendrimer, liposome, protein, and viral envelop.

30. The signal-activated polynucleotide construct of claim 29, wherein said chemical modifications comprise chemical attachments to an 5′

-end, an 3′

-end, or an internal nucleotide of the construct.

31. The signal-activated polynucleotide construct of claim 29, wherein said non-covalent association comprise one or more of electrostatic attraction, attachment via salt bridges, hydrogen bonds, polyion-condensation and charge inversion, van der Waals interactions, and hydrophobic/hydrophilic interactions.

44. The signal-activated polynucleotide construct of claim 1, wherein:

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and part of the sense sequence to create two additional duplex regions, wherein one of said additional duplex regions is linked to a single-stranded loop with a nuclease-resistant sequence, and the other said additional duplex regions is linked to a hairpin structure comprising two DNA segments, one on each strand of the hairpin structure, wherein the two DNA segments base pair with each other at about 2 nucleotides;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and disrupts the base pairing between the two DNA segments so as to allow RNase H to cleave an RNA sequence hybridized to one or both of said DNA segments.

8. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein said part of the guide sequence is a continuous stretch of about 3 nucleotides or less.

9. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein said part of the sense sequence is a continuous stretch of about 5 nucleotides or less.

11. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein in the presence of the signal polynucleotides, an extension duplex region extends said duplex region formed by said guide sequence and said sense sequence.

12. The signal-activated polynucleotide construct of claim 11, wherein said extension duplex region comprises one or more mismatches.

13. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein in the absence of the signal polynucleotides, a first part of the signal detecting sequence hybridizes with said region comprising said part of the guide sequence to form a first additional duplex region.

14. The signal-activated polynucleotide construct of claim 13, wherein a second part of the signal detecting sequence hybridizes with said regions comprising said part of the sense sequence to form a second additional duplex region.

15. The signal-activated polynucleotide construct of claim 14, wherein neither said first additional duplex region nor said second additional duplex region triggers PKR-response in a mammalian cell.

16. The signal-activated polynucleotide construct of claim 14, wherein the guide sequence and the sense sequence form a short duplex region of no more than 19 base pairs in length, and wherein the combined length of any two of said short duplex region, said first additional duplex region, and said second additional duplex region is no more than 29 base pairs in length.

17. The signal-activated polynucleotide construct of claim 14, wherein said first additional duplex region and/or said second additional duplex region are more than 20 base pairs in length, and comprise wobble base pairing or mismatch in every 4-8 nucleotides.

18. The signal-activated polynucleotide construct of claim 14, further comprising one or more 2′

-o-methyl modifications that inhibit PKR and/or TLR activation.

19. The signal-activated polynucleotide construct of claim 14, further comprising one or more single-stranded segments and/or additional duplexes around:

(1) the three-way junction region, (2) said first additional duplex region, and/or (3) said second additional duplex region.

20. The signal-activated polynucleotide construct of claim 19, wherein said single-stranded segments and/or additional duplexes comprise non-canonical base-pairings or chelation of poly-valent cations.

21. The signal-activated polynucleotide construct of claim 13, wherein one end of the first additional duplex region comprises a first unstructured single-stranded region.

22. The signal-activated polynucleotide construct of claim 14, wherein one end of the second additional duplex region comprises a second unstructured single-stranded region.

23. The signal-activated polynucleotide construct of claim 22, wherein said first unstructured single-stranded region and/or said second unstructured single-stranded region comprise modified bases that inhibit endonuclease degradation.

26. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal-activated polynucleotide construct comprises one or more chemical modifications that are largely/mostly present on the sense sequence but are absent on the guide sequence.

32. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides comprise an mRNA, an miRNA, an siRNA, a ribozyme, or a non-coding RNA.

36. The signal-activated polynucleotide construct of claim 32, wherein the mRNA is not a transcript of the target gene.

33. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides comprise two independent/unrelated signal polynucleotides.

34. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein at least one of said signal polynucleotides is not a transcript of the target gene.

35. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the target gene is a wild-type gene with normal expression.

37. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides are within a 3′

-UTR region.

38. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides are within an evolutionary conserved site for miRNA binding.

39. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal polynucleotides comprise a viral RNA, or mutations associated with a disease or cellular process.

40. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and part of the sense sequence, and comprises a 3-4 nucleotide single-stranded region between the sequences that hybridize to said part of the guide sequence and said part of the sense sequence.

41. A signal-activated polynucleotide construct, comprising:

(1) a guide sequence and a sense sequence capable of forming a duplex region that can either become, or be cleaved by Dicer or RNase III to generate, an siRNA or an miRNA comprising the guide sequence, wherein the guide sequence is substantially complementary to a transcript of a target gene; and

,

(2) a signal detecting sequence capable of hybridizing with;

(a) one or more signal polynucleotides that are not linked to said signal-activated polynucleotide construct, and,

(b) one or more regions comprising part of the guide sequence and/or part of the sense sequence;

wherein,

(i) in the absence of the signal polynucleotides, the signal detecting sequence hybridizes with said one or more regions comprising part of the guide sequence and/or part of the sense sequence, wherein generation of the siRNA or miRNA is inhibited, and/or productive incorporation of said siRNA or miRNA into RISC is inhibited;

(ii) in the presence of the signal polynucleotides, the signal detecting sequence hybridizes with said signal polynucleotides, and allows the formation of said duplex region for the generation of said siRNA or said miRNA, and,

wherein two or more signal polynucleotides bind simultaneously or sequentially to different portions of said signal detecting sequence.

42. The signal-activated polynucleotide construct of claim 41, wherein the presence of at least two of said signal polynucleotides are required for the generation of said siRNA or miRNA.

43. The signal-activated polynucleotide construct of claim 41, wherein the presence of one of said two or more signal polynucleotides is sufficient for the generation of said siRNA or miRNA.