Genes regulating plant branching, promotors, genetic constructs containing same and uses thereof

• US 9,040,776 B2
• Filed: 11/27/2009
• Issued: 05/26/2015
• Est. Priority Date: 01/13/2009
• Status: Active Grant

• Current Assignee: Consejo Superior de Investigaciones Científicas,
• Original Assignee: Consejo Superior de Investigaciones Científicas,
• Inventors: Martn Trillo, Mar, Rodrguez Buey, Mara Luisa, Cubas Domnguez, Pilar
• Primary Examiner(s): Mehta, Ashwin
• Assistant Examiner(s): COOBS, REBECCA J
• Application Number:
  US13/144,348
• Publication Number:
  US 20120017337A1
• Time in Patent Office: 2,006 Days
• Field of Search: 800/290
• US Class Current: 800/290
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8226 Stem-specific, e.g. including tubers, beets
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The invention relates to genes coding for TCP family transcription factors and having a biological role in the development of axillary buds and branch growth. Furthermore, the invention relates to the promoters of the transcription of said genes, to the genetic constructs containing same and to the uses thereof, including the use of agents that modulate the expression of these genes in order to modify plant architecture.

Claims

1. An isolated cDNA molecule capable of being translated into an amino acid sequence, said amino acid sequence comprising:

a) a peptide having at least 95% identity with SEQ ID NO;

2, or

b) the peptide having SEQ ID NO;

2;

wherein said peptide maintains the function of suppressing the development of axillary buds and/or branch elongation in a plant.

2. An isolated cDNA molecule according to claim 1 operatively linked to a regulatory expression sequence of a gene of interest in the axillary meristem of a plant, wherein:

a) the regulatory expression sequence comprises at least 95% identity with SEQ ID NO;

6, or

b) the regulatory expression sequence is SEQ ID NO;

6.

6. A method to reduce the number of branches of a plant with respect to a control plant, comprising:

a) transfecting the isolated cDNA molecule according to claim 1, or a vector that comprises said cDNA,molecule or a vector comprising a regulatory expression sequence operatively linked to said cDNA molecule, wherein the regulatory expression sequence comprises at least 95% identity with SEQ ID NO;

6, or the regulatory expression sequence is SEQ ID NO;

6, in a plant cell or culture of host plant cells, and

(b) growing the transfected plant cell or culture of host plant cells in a suitable medium, until regenerating a complete plant.

7. The method according to claim 6, wherein the transfected cell can be taxonomically classified as belonging to the species Solanum lycopersicum.

3. A vector comprising:

(a) an isolated cDNA molecule capable of being translated into an amino acid sequence said amino acid sequence comprising;

(i) a peptide having at least 95% identity with SEQ ID NO;

2, or

(ii) the peptide having SEQ ID NO;

2, wherein said peptide maintains the function of suppressing the development of axillary buds and branch elongation;

or

(b) a regulatory expression sequence operatively linked to the cDNA molecule according to (a), wherein;

(i) the regulatory expression sequence comprises at least 95% identity with SEQ ID NO;

6, or

(ii) the regulatory expression sequence is SEQ ID NO;

6.

4. A plant, a seed, a plant cell, a part of the plant or a grain of pollen comprising the vector according to claim 3 (a) or (b).

5. The plant, a seed, a plant cell, a part of the plant or a grain of pollen according to claim 4, which can be taxonomically classified as belonging to the species Solanum lycopersicum.

8. A method for obtaining a plant having an increased number of branches with respect to a control plant comprising:

(a) obtaining plant material from a plant (parent) which can be taxonomically classified as belonging to the species Solanum lycopersicum;

(b) subjecting the plant material of step (a) to a mutagenesis process;

(c) culturing the mutated plant material until regenerating a complete plant, and its descendants;

(d) analysing the descendants of the plants of step (c) to detect at least one loss of function mutation in at least one copy of a polynucleotide encoding a peptide having at least 95% identity with SEQ ID NO;

2 or the peptide having SEQ ID NO;

2;

(e) selecting the descendants of the mutated plant with said at least one mutation in at least one copy of said polynucleotide; and

(f) optionally, culturing the plant selected to obtain further descendants with said at least one mutation.