High amylose wheat

  • US 9,060,533 B2
  • Filed: 11/04/2011
  • Issued: 06/23/2015
  • Est. Priority Date: 11/04/2010
  • Status: Active Grant
First Claim
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1. Wheat grain (Triticum aestivum) comprising an embryo, an endosperm, starch and a reduced level or activity of total SBEII protein, wherein the embryo comprises a loss of function mutation in alleles of endogenous genes of SBEIIa-A, SBEIIa-B, SBEIIa-D, SBEIIb-A, SBEIIb-B or SBEIIb-D, such that the level or activity of total SBEII protein in the grain is between 2% and 30% of the level or activity of total SBEII protein in a wild-type wheat grain, whereini) said alleles include 2, 4 or 6 SBEIIb alleles which are null alleles and 5 or 6 SBEIIa alleles which each comprise a loss of function mutation, wherein at least one of the 5 or 6 SBEIIa alleles which comprises a loss of function mutation comprises a loss of function point mutation;

  • ii) the grain has a germination rate of between about 70% and about 100% relative to the germination rate of a wild-type grain, andiii) the starch of the grain has an amylose content of at least 50% (w/w) as determined by an iodometric method.

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