Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of HIV-1

  • US 9,074,262 B2
  • Filed: 03/27/2014
  • Issued: 07/07/2015
  • Est. Priority Date: 08/08/1997
  • Status: Expired due to Fees
First Claim
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1. A method for amplifying HIV-1 nucleic acid in a sample, comprising:

  • a) contacting the sample with a pair of oligonucleotide primers that bind to a first primer binding site and a second primer binding site located within the LTR region of the HIV-1 genome; and

    b) performing a nucleic acid amplification under conditions wherein said oligonucleotide primers bind only to said first and second primer binding sites, thereby amplifying HIV-1 nucleic acid in the sample;

    wherein said pair of oligonucleotide primers consists of a first primer and a second primer,wherein said first primer consists essentially of a first oligonucleotide that is fully complementary to a sequence of the LTR region at the first primer binding site, said oligonucleotide being 15-26 nucleotides in length and comprising at least 15 sequential nucleotides of the nucleotide sequence of;

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