Methods for controlling the galactosylation profile of recombinantly-expressed proteins

  • US 9,090,688 B2
  • Filed: 09/22/2014
  • Issued: 07/28/2015
  • Est. Priority Date: 04/27/2011
  • Status: Active Grant
First Claim
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1. A process for producing an antibody comprising the heavy and light chain variable domains of adalimumab, the process comprising culturing a mammalian cell which produces said antibody in a cell culture media that comprises a concentration of manganese of 0.2-100 μ

  • M and a concentration of galactose of 1-100 mM, wherein said concentration of manganese and galactose is sufficient to increase the galactosylation level of said antibody as compared to the galactosylation level of an antibody comprising the heavy and light chain variable domains of adalimumab produced in a cell culture media that does not comprise said concentration of manganese and galactose, thereby producing said antibody.

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