Yeast strains and method for lignocellulose to ethanol production

• US 9,102,931 B1
• Filed: 08/01/2012
• Issued: 08/11/2015
• Est. Priority Date: 08/01/2012
• Status: Active Grant

• Current Assignee: United States Department Of Agriculture
• Original Assignee: United States Department Of Agriculture
• Inventors: Liu, Zonglin L., Slininger, Patricia J., Moon, Jaewoong, Ma, Menggen
• Primary Examiner(s): Pak, Yong
• Application Number:
  US13/563,868
• Time in Patent Office: 1,105 Days
• US Class Current: 1/1
• CPC Class Codes: C12N 15/79 Vectors or expression systems specially adapted for eukaryotic hosts
• CPC Class Codes: C12N 15/81 for yeasts
• CPC Class Codes: C12N 9/90 Isomerases (5.)
• CPC Class Codes: C12N 9/92 Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12Y 503/01005 Xylose isomerase (5.3.1.5)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Disclosed is a method to incorporate xylose transport related genes into a yeast strain for lignocelluloses to ethanol production. More specifically, the invention relates to novel Saccharomyces cerevisiae strains NRRL Y-50463 and yeast strains having novel xylose transporter genes, the genes deposited as GenBank JF343555, GenBank JF343556, GenBank JF343557, GenBank JF343558, and GenBank JF343559. The yeast strains having said genes are deposited as Y-50465, Y-50466, Y-50746, Y-50747, Y-50748, and Y-50749.

Claims

1. A recombinant yeast strain comprising a nucleotide sequence encoding a xylose isomerase gene and a nucleotide sequence encoding xylose transporter gene, wherein the xylose isomerase gene is SEQ ID NO 63 and the xylose transporter gene is selected from the group consisting of SEQ. ID. Nos. 64, 65, 66, 67, 68, or 69.

4. A method of producing ethanol from the fermentation of xylose comprising:

culturing the yeast strain of claim 1 in xylose-containing material under suitable conditions for a period of time sufficient to allow fermentation of at least a portion of the xylose to ethanol.

5. The method of claim 4, wherein the xylose-containing material further comprises glucose.

2. A recombinant yeast strain having yeast strain Y-50049 as a parent strain comprising a nucleotide sequence encoding a xylose isomerase gene and a nucleotide sequence encoding xylose transporter gene, wherein the xylose isomerase gene is SEQ ID NO 63 and the xylose transporter gene is selected from the group consisting of SEQ. ID. Nos. 64, 65, 66, 67, 68, or 69.

3. A yeast strain deposited as NRRL Y-50465, NRRL Y-50466, NRRL Y-50746, NRRL Y-50747, NRRL Y-50748 or NRRL Y-50749.