Nucleic acid sequences encoding transcription factors regulating alkaloid biosynthesis and their use in modifying plant metabolism
First Claim
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1. A method for increasing a nicotinic alkaloid in a Nicotiana plant, comprising growing the plant under conditions which allow for the expression of a transcription factor from an exogenous nucleic acid sequence in the plant, wherein the exogenous nucleic acid sequence is introduced using a vector comprising a nucleotide sequence selected from the group consisting of:
- (i) the nucleotide sequence set forth in SEQ ID NO;
5 or SEQ ID NO;
6;
(ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
7;
(iii) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (i) or (ii), and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis; and
(iv) a nucleotide sequence that hybridizes under high stringency conditions to the complement of the nucleotide sequences of (i), (ii), and/or (iii), and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis;
wherein expression of the transcription factor results in the plant having an increased nicotinic alkaloid content as compared to a control plant grown under similar conditions.
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Abstract
Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
23 Citations
24 Claims
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1. A method for increasing a nicotinic alkaloid in a Nicotiana plant, comprising growing the plant under conditions which allow for the expression of a transcription factor from an exogenous nucleic acid sequence in the plant, wherein the exogenous nucleic acid sequence is introduced using a vector comprising a nucleotide sequence selected from the group consisting of:
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(i) the nucleotide sequence set forth in SEQ ID NO;
5 or SEQ ID NO;
6;(ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
7;(iii) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (i) or (ii), and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis; and (iv) a nucleotide sequence that hybridizes under high stringency conditions to the complement of the nucleotide sequences of (i), (ii), and/or (iii), and which encodes a transcription factor that positively regulates nicotinic alkaloid biosynthesis; wherein expression of the transcription factor results in the plant having an increased nicotinic alkaloid content as compared to a control plant grown under similar conditions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Current Assignee22nd Century Limited, LLC (22nd Century Group, Inc.)
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Original Assignee22nd Century Limited, LLC (22nd Century Group, Inc.)
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InventorsPage, Jonathan, Todd, Andrea T.
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Primary Examiner(s)Kallis, Russell
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Application NumberUS14/261,203Publication NumberTime in Patent Office509 DaysField of Search800/278US Class Current1/1CPC Class CodesA24B 13/00 Tobacco for pipes, for ciga...A24B 15/10 Chemical features of tobacc...A24B 15/12 of reconstituted tobaccoA24D 1/00 Cigars; CigarettesA24D 1/10 with extinguishersC07K 14/415 from plantsC07K 14/4702 Regulators; Modulating acti...C12N 15/01 Preparation of mutants with...C12N 15/8213 Targeted insertion of genes...C12N 15/8218 Antisense, co-suppression, ...C12N 15/8243 involving biosynthetic or m...
