×

Method for increasing a nicotinic alkaloid in a;plant by introducing a mutation into the gene encoding the NbTF7 transcription factor and plants and products made by said method

  • US 9,175,302 B2
  • Filed: 04/24/2014
  • Issued: 11/03/2015
  • Est. Priority Date: 05/25/2007
  • Status: Active Grant
First Claim
Patent Images

1. A method for increasing a nicotinic alkaloid in a Nicotiana plant, comprising:

  • (a) genetically engineering a population of plant cells to eliminate production of a protein encoded by the nucleotide sequence set forth in SEQ ID NO;

    14, comprising introducing one or more mutations into the region of SEQ ID NO;

    14 that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;

    16; and

    (b) detecting and selecting a target mutated plant cell or a plant derived from such a cell, wherein the target mutated plant cell or plant has a mutation in a gene encoding a transcription factor that negatively regulates nicotinic alkaloid biosynthesis and wherein the targeted plant cell or plant has an increased nicotinic alkaloid content as compared to a control plant cell or plant.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×