Method for increasing a nicotinic alkaloid in a;plant by introducing a mutation into the gene encoding the NbTF7 transcription factor and plants and products made by said method
First Claim
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1. A method for increasing a nicotinic alkaloid in a Nicotiana plant, comprising:
- (a) genetically engineering a population of plant cells to eliminate production of a protein encoded by the nucleotide sequence set forth in SEQ ID NO;
14, comprising introducing one or more mutations into the region of SEQ ID NO;
14 that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
16; and
(b) detecting and selecting a target mutated plant cell or a plant derived from such a cell, wherein the target mutated plant cell or plant has a mutation in a gene encoding a transcription factor that negatively regulates nicotinic alkaloid biosynthesis and wherein the targeted plant cell or plant has an increased nicotinic alkaloid content as compared to a control plant cell or plant.
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Abstract
Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis. The present invention is directed to a method for increasing a nicotinic alkaloid in a nicotiana plant by introducing a mutation into the gene encoding the NbTF7 transcription factor. The present invention is also directed to mutagenized plants produced by this method and products made from these plants.
23 Citations
15 Claims
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1. A method for increasing a nicotinic alkaloid in a Nicotiana plant, comprising:
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(a) genetically engineering a population of plant cells to eliminate production of a protein encoded by the nucleotide sequence set forth in SEQ ID NO;
14, comprising introducing one or more mutations into the region of SEQ ID NO;
14 that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO;
16; and(b) detecting and selecting a target mutated plant cell or a plant derived from such a cell, wherein the target mutated plant cell or plant has a mutation in a gene encoding a transcription factor that negatively regulates nicotinic alkaloid biosynthesis and wherein the targeted plant cell or plant has an increased nicotinic alkaloid content as compared to a control plant cell or plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification