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Kits and methods for processing stem cells from bone marrow or umbilical cord blood

  • US 9,241,959 B2
  • Filed: 03/10/2014
  • Issued: 01/26/2016
  • Est. Priority Date: 03/13/2013
  • Status: Active Grant
First Claim
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1. A method for separating stem cells using a kit for processing stem cells from bone marrow or umbilical cord blood comprisinga precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate;

  • anda separation reagent comprising an aqueous solution comprising Ficoll polysaccharide and diatrizoate and having a density of 1.0-1.2 g/ml, which method comprises;

    a) providing a bone marrow or umbilical cord blood sample;

    b) adding the precipitation reagent to the bone marrow or umbilical cord blood sample, mixing and letting the solution settle, followed by removal and centrifugation of the upper layer to obtain a concentrated sample; and

    c) adding the concentrated sample from step b) on top of the separation reagent and performing density gradient centrifugation to obtain a layer comprising the stem cells.

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