Kits and methods for processing stem cells from bone marrow or umbilical cord blood
First Claim
1. A method for separating stem cells using a kit for processing stem cells from bone marrow or umbilical cord blood comprisinga precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate;
- anda separation reagent comprising an aqueous solution comprising Ficoll polysaccharide and diatrizoate and having a density of 1.0-1.2 g/ml, which method comprises;
a) providing a bone marrow or umbilical cord blood sample;
b) adding the precipitation reagent to the bone marrow or umbilical cord blood sample, mixing and letting the solution settle, followed by removal and centrifugation of the upper layer to obtain a concentrated sample; and
c) adding the concentrated sample from step b) on top of the separation reagent and performing density gradient centrifugation to obtain a layer comprising the stem cells.
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Abstract
Provided herein are kits for processing stem cells from bone marrow or umbilical cord blood, comprising: a) a precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate; and b) a separation reagent comprising an aqueous solution comprising Ficoll and diatrizoate and having a density of 1.0-1.2 g/ml, and methods of use. Further provided herein is a collection of stem cells obtained by the kits and methods disclosed herein comprising stem cells from bone marrow or umbilical cord blood, and uses thereof.
13 Citations
26 Claims
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1. A method for separating stem cells using a kit for processing stem cells from bone marrow or umbilical cord blood comprising
a precipitation reagent comprising an aqueous solution of 0.1-30% hydroxyethyl starch or 0.1-30% methyl cellulose, and 0.1-20% of cerebroprotein hydrolysate; - and
a separation reagent comprising an aqueous solution comprising Ficoll polysaccharide and diatrizoate and having a density of 1.0-1.2 g/ml, which method comprises; a) providing a bone marrow or umbilical cord blood sample; b) adding the precipitation reagent to the bone marrow or umbilical cord blood sample, mixing and letting the solution settle, followed by removal and centrifugation of the upper layer to obtain a concentrated sample; and c) adding the concentrated sample from step b) on top of the separation reagent and performing density gradient centrifugation to obtain a layer comprising the stem cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification