Enzyme triggered redox altering chemical elimination (E-TRACE) immunoassay

• US 9,250,234 B2
• Filed: 01/19/2012
• Issued: 02/02/2016
• Est. Priority Date: 01/19/2011
• Status: Expired due to Fees

• Current Assignee: Ohmx Corporation
• Original Assignee: Ohmx Corporation
• Inventors: Bertin, Paul A., Ahrens, Michael J.
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US13/354,200
• Publication Number:
  US 20120181186A1
• Time in Patent Office: 1,475 Days
• Field of Search: 205/777.5, 205/792, 436 24- 34, 436/74, 436/83, 436/84
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms

Abstract

Described are methods for detecting a target analyte in a test sample by providing a solid support comprising an electrode comprising: (i) a self-assembled monolayer (SAM), (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰; then contacting the target analyte and said solid support under conditions wherein said target analyte reacts with a peroxide generating enzyme to generate peroxide and said self-immolative moiety is removed such that said EAM has a second E⁰ test sample; and then detecting said second E⁰ as an indication of the presence of said target analyte. Also provided are compositions for use in the preceding methods.

Claims

1. A method for detecting a target analyte in a test sample, said method comprising:

(a) providing a solid support comprising an electrode comprising;

(i) a self-assembled monolayer (SAM),

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰, and

(b) contacting said target analyte and said solid support under conditions wherein said target analyte reacts with a peroxide generating enzyme to generate peroxide and said self-immolative moiety is removed such that said EAM has a second E⁰, wherein said peroxide generating enzyme is immobilized or physisorbed onto the solid support, the electrode, or SAM, or wherein said peroxide generating enzyme is in a solution in contact with the electrode; and

(c) detecting said second E⁰ as an indication of the presence of said target analyte.

2. The method according to claim 1, wherein said target analyte is NADH and said peroxide generating moiety is NADH oxidase (NAOX).

3. The method according to claim 1, wherein said solid support comprises an array of electrodes.

4. The method according to claim 1, wherein said transition metal is selected from the group consisting of iron, ruthenium and osmium.

5. The method according to claim 1, wherein said EAM is a ferrocene.

6. The method according to claim 1, wherein said detecting is done amperommetrically at each first and second E⁰.

7. A method for detecting a target analyte in a test sample, said method comprising:

(a) providing a solid support comprising an electrode comprising;

(i) a self-assembled monolayer (SAM),

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰, and

(b) contacting said target analyte and said solid support in the presence of an intermediary enzyme and an additional substrate for said enzyme, under conditions wherein if said analyte is present, the target analyte and said additional substrate reacts with the intermediary enzyme to form a first complex;

(c) contacting said target analyte and said solid support in the presence of the intermediary enzyme and the additional substrate for said enzyme with a peroxide generating enzyme under conditions wherein if a first complex is formed, said first complex reacts with said peroxide generating enzyme to generate peroxide and said self-immolative moiety is removed such that said EAM has a second E⁰, wherein said intermediary enzyme is immobilized or physisorbed onto the solid support, the electrode, or SAM, or wherein said intermediary enzyme is in a solution in contact with the electrode; and

(d) detecting said second E⁰ as an indication of the presence of said target analyte.

8. The method according to claim 7, wherein steps (b) and (c) are carried out separately.

9. The method according to claim 7, wherein steps (b) and (c) are carried out simultaneously.

10. The method according to claim 7, wherein said target analyte is ATP, said intermediary enzyme is glycerol kinase and said substrate of said intermediary enzyme is glycerol, and said peroxide generating moiety is glycerol-3-oxidase.

11. A composition comprising a solid support comprising:

(a) an electrode comprising;

(i) a self-assembled monolayer (SAM);

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰ when said self-immolative moiety is covalently attached to said EAM and a second E⁰ when said self-immolative moiety is absent; and

(iii) a capture binding ligand comprising a substrate of an ATP-dependent intermediary enzyme; and

(b) a soluble capture ligand comprising a peroxide generating moiety,

wherein an intermediary enzyme is immobilized or physisorbed onto the solid support, the electrode, or SAM.

12. A composition comprising a solid support comprising:

an electrode comprising;

(i) a self-assembled monolayer (SAM);

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰ when said self-immolative moiety is covalently attached to said EAM and a second E⁰ when said self-immolative moiety is absent; and

(iii) a peroxide generating enzyme,

wherein said peroxide generating enzyme is immobilized or physisorbed onto the solid support, the electrode, or SAM.

13. A composition comprising:

(I) a solid support comprising;

(a) an electrode comprising;

(i) a self-assembled monolayer (SAM);

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰ when said self-immolative moiety is covalently attached to said EAM and a second E⁰ when said self-immolative moiety is absent; and

(iii) a capture binding ligand comprising a substrate of an ATP-dependent intermediary enzyme; and

(b) a soluble capture ligand comprising a peroxide generating moiety; and

(II) an intermediary enzyme in a solution in contact with the electrode.

14. A composition comprising:

(a) a solid support comprising;

an electrode comprising;

(i) a self-assembled monolayer (SAM); and

(ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E⁰ when said self-immolative moiety is covalently attached to said EAM and a second E⁰ when said self-immolative moiety is absent; and

(b) a peroxide generating enzyme, wherein said peroxide generating enzyme is in a solution in contact with the electrode.