Analysis, secure access to, and transmission of array images

• US 9,251,583 B2
• Filed: 03/10/2014
• Issued: 02/02/2016
• Est. Priority Date: 11/15/2002
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Seul, Michael, Xia, Xiongwu, Chau, Chiu, Determan, Scott
• Primary Examiner(s): TUCKER, WESLEY J
• Application Number:
  US14/202,357
• Publication Number:
  US 20140369579A1
• Time in Patent Office: 694 Days
• Field of Search: 382/128, 382/129, 382/232, 382/294
• US Class Current: 1/1
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
• CPC Class Codes: G06F 18/00 Pattern recognition
• CPC Class Codes: G06T 2207/10064 Fluorescence image
• CPC Class Codes: G06T 2207/20152 Watershed segmentation
• CPC Class Codes: G06T 2207/30072 Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06T 7/11 Region-based segmentation
• CPC Class Codes: G06T 7/155 involving morphological operators
• CPC Class Codes: G06T 7/33 using feature-based methods
• CPC Class Codes: G06V 20/698 Matching; Classification
• CPC Class Codes: G06V 2201/04 Recognition of patterns in DNA microarrays

Abstract

Systems and methods are provided the autocentering, autofocusing, acquiring, decoding, aligning, analyzing and exchanging among various parties, images, where the images are of arrays of signals associated with ligand-receptor interactions, and more particularly, ligand-receptor interactions where a multitude of receptors are associated with microparticles or microbeads. The beads are encoded to indicate the identity of the receptor attached, and therefore, an assay image and a decoding image are aligned to effect the decoding. The images or data extracted from such images can be exchanged between de-centralized assay locations and a centralized location where the data are analyzed to indicate assay results. Access to data can be restricted to authorized parties in possession of certain coding information, so as to preserve confidentiality.

Claims

1. A method of conducting an assay and analyzing results therefrom at separate locations, comprising:

conducting an assay on a patient sample by contacting a uniquely encoded array of receptors with the patient sample and determining if the patient sample comprises any ligands that interact with the receptors by analyzing an assay image for optical signatures that are associated with receptor-ligand interactions,

recording the assay image in a first processor, and

decoding the array with a second processor at a location other than the location at which the assay is conducted, wherein the first and second processors communicate by way of a data network,

wherein the array is decoded by segmenting an image of the array, wherein information about the array geometry is known, said segmenting comprising creating a reference grid based on a brightfield image of the array such that each individual field of the grid includes a single object and the outer dimension of the grid corresponds to the dimensions of the array, and calculating an optical signal for each individual grid field, wherein the signal is characteristic of the object contained in said field.

2. The method of claim 1, wherein the uniquely encoded array is an array of encoded beads, wherein different beads have different receptors associated therewith.

3. The array of claim 2, wherein the encoding of the beads is with different colors.

4. The array of claim 1, wherein the optical signatures from the receptors result from the association of a fluorescent entity with the ligand attached to the receptor.

5. The method of claim 1, further comprising obtaining the patient sample, wherein the obtaining and conducting steps are conducted at separate locations.

6. The method of claim 1, further including the step of providing the one or more images of the uniquely encoded array of receptors recorded before conducting the assay to the location at which the assay was conducted, to the patient, or to another location.

7. The method of claim 1, further including the step of providing the one or more images of the uniquely encoded array of receptors recorded after conducting the assay to the location at which the assay was conducted, to the patient, or to another location.

8. The method of claim 1, wherein the assay relates to patient genetic information or to information about certain proteins or antibodies in a patient.

9. The method of claim 1, wherein the assay relates to the presence of tumor markers, markers associated with autoimmune disorders, blood-borne viruses, therapeutic drugs, genetic mutations associated with genetic diseases, polymorphisms, or other chromosomal abnormalities in the patient sample.

10. The method of claim 1, wherein the assay is a multiplexed assay.

11. The method of claim 1, further including the step of transmitting the assay image, one or more images of the uniquely encoded array of receptors, or both to a location where the images are archived.

12. The method of claim 1, wherein the identity of the patient from whom the patient sample is obtained is available only to authorized parties.

13. The method of claim 11, wherein all images are transmitted with an encryption key so that an image can be accessed only by an authorized party with access to the encryption key.

14. The method of claim 13, wherein the encryption key is a pattern of the encoded array of receptors.

15. The method of claim 13, wherein the encryption key is a bar code or other unique identifier code.