Conjugation process of bacterial polysaccharides to carrier proteins

  • US 9,265,839 B2
  • Filed: 03/10/2014
  • Issued: 02/23/2016
  • Est. Priority Date: 03/09/2010
  • Status: Active Grant
First Claim
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1. A process for conjugating a bacterial saccharide and reducing the sizing effect on bacterial saccharide comprising the steps ofa) reacting the bacterial saccharide with 0.001-0.7 molar equivalents of periodate to form an activated bacterial saccharide;

  • b) mixing the activated bacterial saccharide with a carrier protein;

    c) reacting the activated bacterial saccharide and the carrier protein with a reducing agent to form a conjugate;

    wherein step a) occurs in a buffer which does not contain an amine group, and the buffer has a concentration between 1-100 mM and wherein the bacterial saccharide is S. pneumoniae capsular saccharide 6B.

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