Soybean polymorphisms and methods of genotyping

• US 9,271,455 B2
• Filed: 05/29/2008
• Issued: 03/01/2016
• Est. Priority Date: 05/31/2007
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Wu, Kunsheng, LeDeaux, John, Butruille, David, Gupta, Anju, Eathington, Sam, Bull, Jason, Edwards, Marlin, McLaird, Paul, Johnson, Richard
• Primary Examiner(s): ROBINSON, KEITH O NEAL
• Application Number:
  US12/601,461
• Publication Number:
  US 20100275286A1
• Time in Patent Office: 2,833 Days
• Field of Search: 800/267, 800/312
• US Class Current: 1/1
• CPC Class Codes: A01H 1/045 using molecular markers

Abstract

Polymorphic soybean DNA loci useful for genotyping between at least two varieties of soybean. Sequences of the loci are useful for providing the basis for designing primers and probe oligonucleotides for detecting polymorphisms in soybean DNA. Polymorphisms are useful for genotyping applications in soybean. The polymorphic markers are useful to establish marker/trait associations, e.g. in linkage disequilibrium mapping and association studies, positional cloning and transgenic applications, marker-aided breeding and marker-assisted selection, hybrid prediction and identity by descent studies. The polymorphic markers are also useful in mapping libraries of DNA clones, e.g. for soybean QTLs and genes linked to polymorphisms.

Claims

1. A method of genotyping a soybean plant to select a parent plant, a progeny plant or a tester plant with increased yield for breeding, said method comprising the steps of:

a. obtaining a DNA or RNA sample from a tissue of at least one soybean plant;

b. determining the allelic state of the soybean genomic DNA polymorphism of SEQ ID NO;

3122 associated with increased yield, for said sample from step (a); and

c. using said determination of the allelic state of the soybean genomic DNA polymorphism of SEQ ID NO;

3122 of step (b) to select a parent plant, a progeny plant or a tester plant with increased yield for breeding.

2. The method according to claim 1, wherein the soybean genomic DNA polymorphism of SEQ ID NO:

3122 is a first polymorphism used to identify plants for breeding with increased yield and wherein the allelic state of one or more additional soybean genomic polymorphisms selected from the group consisting of SEQ ID NO;

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, 80, 88, 980, 538, 1925, 3669, 2270, 1397, 3747, 888, 365, 2132, 1972, 459, 762, and SEQ ID NO;

1094, associated with increased yield, is also determined to select a parent plant, a progeny plant, or a tester plant with increased yield for breeding.

3. The method according to claim 2, wherein the allelic state of at least seven or more soybean genomic polymorphisms selected from the group consisting of SEQ ID NO:

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, 80, 88, 980, 538, 1925, 3669, 2270, 1397, 3747, 888, 365, 2132, 1972, 459, 762, and SEQ ID NO;

1094, associated with increased yield, is also determined to select a parent plant, a progeny plant, or a tester plant with increased yield for breeding.

4. A method of genotyping a soybean plant to select a parent plant, a progeny plant or a tester plant with increased yield for breeding, said method comprising the steps of:

a. obtaining a DNA or RNA sample from a tissue of at least one soybean plant;

b. determining the allelic state of a set of soybean genomic DNA polymorphisms comprising the soybean genomic DNA polymorphism of SEQ ID NO;

3122 associated with increased yield, and one or more additional polymorphisms selected from the group consisting of SEQ ID NO;

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, 80, 88, 980, 538, 1925, 3669, 2270, 1397, 3747, 888, 365, 2132, 1972, 459, 762, and 1094, associated with increased yield, for said sample from step (a), wherein said allelic state is determined with a set of nucleic acid molecules that provide for typing of said soybean genomic DNA polymorphisms; and

c. using said determination of the allelic state of the set of soybean genomic DNA polymorphisms comprising the soybean genomic DNA polymorphism of SEQ ID NO;

3122 associated with increased yield, and one or more additional polymorphisms selected from the group consisting of SEQ ID NO;

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, 80, 88, 980, 538, 1925, 3669, 2270, 1397, 3747, 888, 365, 2132, 1972, 459, 762, and 1094, associated with increased yield, to select a parent plant, a progeny plant or a tester plant with increased yield for breeding.

5. The method of genotyping a soybean plant of claim 4, wherein said set of soybean genomic DNA polymorphisms comprises the soybean genomic DNA polymorphism of SEQ ID NO:

3122 associated with increased yield, and at least four or more additional polymorphisms selected from the group consisting of SEQ ID NO;

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, 80, 88, 980, 538, 1925, 3669, 2270, 1397, 3747, 888, 365, 2132, 1972, 459, 762, and 1094, associated with increased yield.

6. The method of genotyping a soybean plant of claim 4, wherein said set of soybean genomic DNA polymorphisms comprises the soybean genomic DNA polymorphism of SEQ ID NO:

3122 associated with increased yield, and at least one or more additional polymorphisms selected from the group consisting of SEQ ID NO;

2914, 3984, 3608, 1448, 69, 1261, 3436, 1142, and 80, associated with increased yield.