Gut flora-derived extracellular vesicles, and method for searching for a disease model, vaccine, and candidate drug and for diagnosis using the same

  • US 9,274,109 B2
  • Filed: 07/02/2014
  • Issued: 03/01/2016
  • Est. Priority Date: 09/01/2009
  • Status: Active Grant
First Claim
Patent Images

1. A method of assessing a subject'"'"'s susceptibility to sepsis associated with a pathogenic strain of E. coli or Klebsiella spp., the method comprising:

  • providing a database listing pre-identified pathogenic strains of E. coli and Klebsiella spp.;

    obtaining a sample comprising gut flora isolated from a subject;

    processing the sample to obtain a concentrated population of extracellular vesicles derived from microorganisms contained in the gut flora;

    processing at least part of the concentrated population to determine if the concentrated population contains at least one substance of a first one of the pre-identified pathogenic strains of E. coli and Klebsiella spp. listed in the database; and

    once determined that at least one substance is of the first pre-identified pathogenic strains of E. coli and Klebsiella spp., further determining that the subject is potentially susceptible to one or more diseases that are associated with the first pre-identified pathogenic strains of E. coli and Klebsiella spp.,wherein processing at least part of the concentrated population comprises;

    mixing at least part of the concentrated population with a forward primer and a reverse primer for cDNA corresponding to 16S rRNA such that if the concentrated population contains extracellular vesicles of the first pre-identified pathogenic strains of E. coli and Klebsiella spp., the forward and reverse primers would recognize the cDNA, the forward primer comprising the sequence of SEQ ID NO;

    4, the reverse primer comprising the sequence of SEP ID NO;

    5,subjecting the mixture to amplification,processing the amplification product to determine if an nucleotide sequence recognized by the forward and reverse primers has been amplified therein, wherein if at least one nucleotide sequence recognized by the forward and reverse primers has been amplified, the at least one nucleotide sequence is determined to comprise the 16S rRNA is of the first pre-identified pathogenic strains of E. coli and Klebsiella spp.,sequencing the at least one amplified nucleotide sequence, andreferring to the database to determine if the at least one sequenced nucleotide sequence is of the first pre-identified pathogenic strains of E. coli and Klebsiella spp.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×