Use of microfluidic systems in the detection of target analytes using microsphere arrays
First Claim
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1. A method of sequencing a target nucleic acid comprising:
- providing a fluidics device comprising;
an inlet in fluid communication a first channel,a detection module in fluid communication with said first channel, said detection module comprising;
an array of wells comprising a tantalum-containing substrate, wherein the wells are configured to hold a single microsphere, a population of microspheres comprising at least a first and a second subpopulation distributed in said wells, wherein each subpopulation comprises a nucleic acid primer, anda second channel in fluid communication with said detection module and an outlet;
contacting the target nucleic acid with the nucleic acid primer; and
sequencing the target nucleic acid.
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Abstract
The invention relates generally to methods and apparatus for conducting analyzes, particularly microfluidic devices for the detection of target analytes.
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16 Claims
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1. A method of sequencing a target nucleic acid comprising:
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providing a fluidics device comprising; an inlet in fluid communication a first channel, a detection module in fluid communication with said first channel, said detection module comprising;
an array of wells comprising a tantalum-containing substrate, wherein the wells are configured to hold a single microsphere, a population of microspheres comprising at least a first and a second subpopulation distributed in said wells, wherein each subpopulation comprises a nucleic acid primer, anda second channel in fluid communication with said detection module and an outlet; contacting the target nucleic acid with the nucleic acid primer; and sequencing the target nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification