Spatial positioning of spectrally labeled beads
First Claim
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1. A multiplex assay detection apparatus, comprising:
- a plurality of spectrally labeled bodies bound to a surface of a solid support,wherein the spectrally labeled bodies are spatially resolved and each comprise a plurality of different fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths;
an excitation energy source configured to excite the plurality of spectrally labeled bodies;
a sensor configured to detect the emission spectrum of each spectrally labeled body; and
a processor configured to utilize machine-readable code including a library of identifiable substances and their associated emission spectra to identify the identifiable substance associated with each spectrally labeled body.
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Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
111 Citations
20 Claims
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1. A multiplex assay detection apparatus, comprising:
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a plurality of spectrally labeled bodies bound to a surface of a solid support, wherein the spectrally labeled bodies are spatially resolved and each comprise a plurality of different fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths; an excitation energy source configured to excite the plurality of spectrally labeled bodies; a sensor configured to detect the emission spectrum of each spectrally labeled body; and a processor configured to utilize machine-readable code including a library of identifiable substances and their associated emission spectra to identify the identifiable substance associated with each spectrally labeled body. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. An assay detection system, comprising:
an assay detection apparatus, comprising; a plurality of spectrally labeled bodies bound to a surface of a solid support, wherein the spectrally labeled bodies are spatially resolved and each comprise a plurality of different fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths; an excitation energy source configured to excite the plurality of spectrally labeled bodies; and a sensor configured to detect the emission spectra of each spectrally labeled body; a computing device in communication with the assay detection apparatus, comprising; a memory for storing machine-readable code including a library of identifiable substances and their associated spectral barcodes, and a processor in communication with the memory and configured to utilize the machine-readable code to identify the identifiable substances associated with the spectrally labeled bodies. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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19. An assay detection method, comprising:
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exciting a plurality of spectrally labeled bodies with an energy source, wherein the spectrally labeled bodies are bound to a surface of a solid support in a spatially resolved configuration and each comprise a plurality of different fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths; detecting emission spectra with a sensor; providing a memory for storing machine-readable code including a library of identifiable substances and their associated emission spectra; and identifying identifiable substances from the emission spectra using a processor, wherein the processor is in communication with the memory and configured to utilize the machine-readable code to identify the identifiable substances associated with the spectrally labeled bodies. - View Dependent Claims (20)
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Specification