Reprogramming cells
First Claim
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1. A method of inducing a non-pluripotent mammalian cell into an induced pluripotent stem cell, the method comprising:
- (a) introducing into the non-pluripotent cell a polynucleotide encoding an Oct4 polypeptide; and
(b) contacting the non-pluripotent cell with a small molecule 3′
-phosphoinositide-dependent kinase-1 (PDK1) activator, a TGFβ
receptor/ALK5 inhibitor, and a MEK inhibitor, thereby inducing the non-pluripotent mammalian cell into an induced pluripotent stem cell.
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Abstract
The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.
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11 Claims
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1. A method of inducing a non-pluripotent mammalian cell into an induced pluripotent stem cell, the method comprising:
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(a) introducing into the non-pluripotent cell a polynucleotide encoding an Oct4 polypeptide; and (b) contacting the non-pluripotent cell with a small molecule 3′
-phosphoinositide-dependent kinase-1 (PDK1) activator, a TGFβ
receptor/ALK5 inhibitor, and a MEK inhibitor, thereby inducing the non-pluripotent mammalian cell into an induced pluripotent stem cell. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A mixture comprising:
- isolated mammalian cells, a small molecule PDK1 activator, and;
(a) a TGFβ
receptor/ALK5 inhibitor;(b) a MEK inhibitor; and (c) one or more vectors comprising a polynucleotide encoding one or more exogenous transcription factors selected from the group consisting of an Oct-4 polypeptide, a Klf-4 polypeptide, a c-myc polypeptide, and a Sox-2 polypeptide. - View Dependent Claims (8, 9, 10, 11)
- isolated mammalian cells, a small molecule PDK1 activator, and;
Specification