Methods and compositions for nucleic acid analysis

  • US 9,347,059 B2
  • Filed: 04/25/2012
  • Issued: 05/24/2016
  • Est. Priority Date: 04/25/2011
  • Status: Active Grant
First Claim
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1. A method comprising:

  • a. generating a plurality of first partitions comprising adaptors, wherein each of the first partitions has on average a first volume and wherein the adaptors comprise unique barcodes, wherein the first partitions are first droplets in a fluid that is immiscible to the first droplets;

    b. generating a plurality of second partitions comprising sample polynucleotides, wherein each of the second partitions has on average a second volume, wherein the second volume is greater than the first volume, wherein the second partitions are second droplets in a immiscible fluid that is immiscible to the second droplets;

    c. applying a treatment to fuse the at least one second partition with at least one first droplet to form a fused partition; and

    d. tagging one of the sample polynucleotides, or fragment thereof, with at least one of the adaptors in the fused partition to form tagged polynucleotides or fragments thereof.

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