Methods and compositions for nucleic acid analysis

  • US 9,347,059 B2
  • Filed: 04/25/2012
  • Issued: 05/24/2016
  • Est. Priority Date: 04/25/2011
  • Status: Active Grant
First Claim
Patent Images

1. A method comprising:

  • a. generating a plurality of first partitions comprising adaptors, wherein each of the first partitions has on average a first volume and wherein the adaptors comprise unique barcodes, wherein the first partitions are first droplets in a fluid that is immiscible to the first droplets;

    b. generating a plurality of second partitions comprising sample polynucleotides, wherein each of the second partitions has on average a second volume, wherein the second volume is greater than the first volume, wherein the second partitions are second droplets in a immiscible fluid that is immiscible to the second droplets;

    c. applying a treatment to fuse the at least one second partition with at least one first droplet to form a fused partition; and

    d. tagging one of the sample polynucleotides, or fragment thereof, with at least one of the adaptors in the fused partition to form tagged polynucleotides or fragments thereof.

View all claims

    Thank you for your feedback