MEMS affinity sensor for continuous monitoring of analytes
First Claim
1. A microdevice for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte, the microdevice comprising:
- a semi-permeable membrane structure;
a substrate; and
a first microchamber and a second microchamber, each formed between the semi-permeable membrane structure and the substrate, each of the first and second microchambers comprising a suspended element positioned to be spaced apart from the substrate;
wherein the suspended element in each of the first and second microchambers comprises a perforated top electrode and the substrate comprises a bottom electrode;
wherein the first microchamber is adapted to receive a solution including the polymer;
wherein the second microchamber is adapted to receive a reference solution for screening effects not caused by the target analyte;
wherein perforations in the top electrode in the first microchamber are configured to allow the solution to fill a gap between the top electrode in the first microchamber and the bottom electrode;
wherein perforations in the top electrode in the second microchamber are configured to allow the reference solution to fill a gap between the top electrode in the second microchamber and the bottom electrode;
wherein the semi-permeable membrane structure is permeable to the target analyte and impermeable to the polymer, thereby when the sample is placed in contact with the semi-permeable membrane structure, the target analyte, if present in the sample, permeates the semi-permeable membrane structure and enters the first microchamber and the second microchamber, respectively, and the polymer is prevented from escaping from the first microchamber through the semi-permeable membrane structure.
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Accused Products
Abstract
Techniques for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte are disclosed. A microdevice useful for the disclosed techniques includes a semi-permeable membrane structure, a substrate, a first and second microchambers formed between the membrane structure and the substrate. The first microchamber can be adapted to receive a solution including the polymer, and the second microchamber can be adapted to receive a reference solution. Environmental target analyte can permeate the semi-permeable membrane structure and enters the first microchamber and the second microchamber. Based on the difference in a property associated with the polymer solution that is responsive to the target analyte-polymer binding, and the corresponding property associated with reference solution, the presence and/or concentration of the target analyte can be determined.
23 Citations
23 Claims
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1. A microdevice for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte, the microdevice comprising:
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a semi-permeable membrane structure; a substrate; and a first microchamber and a second microchamber, each formed between the semi-permeable membrane structure and the substrate, each of the first and second microchambers comprising a suspended element positioned to be spaced apart from the substrate; wherein the suspended element in each of the first and second microchambers comprises a perforated top electrode and the substrate comprises a bottom electrode; wherein the first microchamber is adapted to receive a solution including the polymer; wherein the second microchamber is adapted to receive a reference solution for screening effects not caused by the target analyte; wherein perforations in the top electrode in the first microchamber are configured to allow the solution to fill a gap between the top electrode in the first microchamber and the bottom electrode; wherein perforations in the top electrode in the second microchamber are configured to allow the reference solution to fill a gap between the top electrode in the second microchamber and the bottom electrode; wherein the semi-permeable membrane structure is permeable to the target analyte and impermeable to the polymer, thereby when the sample is placed in contact with the semi-permeable membrane structure, the target analyte, if present in the sample, permeates the semi-permeable membrane structure and enters the first microchamber and the second microchamber, respectively, and the polymer is prevented from escaping from the first microchamber through the semi-permeable membrane structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A microdevice for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte, the microdevice comprising:
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a semi-permeable membrane structure; a substrate; a first microchamber and a second microchamber, each formed between the semi-permeable membrane structure and the substrate, each of the first and second microchambers comprising; a suspended element positioned to be spaced apart from the substrate; and a capacitor formed by a top electrode included in the suspended element and a bottom electrode included in the substrate; wherein the first microchamber is adapted to receive a solution including the polymer; wherein the second microchamber is adapted to receive a reference solution for screening effects not caused by the target analyte; wherein perforations in the top electrode in the first microchamber are configured to allow the solution to fill a gap between the top electrode in the first microchamber and the bottom electrode; wherein perforations in the top electrode in the second microchamber are configured to allow the reference solution to fill a gap between the top electrode in the second microchamber and the bottom electrode; wherein the semi-permeable membrane structure is permeable to the target analyte and impermeable to the polymer, thereby when the sample is placed in contact with the semi-permeable membrane structure, the target analyte, if present in the sample, permeates the semi-permeable membrane structure and enters the first microchamber and the second microchamber, respectively, and the polymer is prevented from escaping from the first microchamber through the semi-permeable membrane structure. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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Specification