Multispecific antibodies comprising full length antibodies and single chain fab fragments
First Claim
Patent Images
1. A multispecific antibody comprisinga) a full length antibody consisting of two antibody heavy chains and two antibody light chains wherein the antibody specifically binds to a first antigen;
- andb) one or two single chain Fab fragments that specifically bind to one or two antigens different from the first antigen, wherein each of the single Fab fragments comprises an antibody light chain variable domain (VL) and an antibody heavy chain variable domain (VH) that binds to the antigen that is different from the first antigen,wherein each of the one or two single chain Fab fragments is fused to the full length antibody via a peptide connector at the C- or N-terminus of the heavy chain of the full length antibody.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to multispecific, especially bispecific antibodies comprising full length antibodies and single chain Fab fragments, methods for their production, pharmaceutical compositions containing the antibodies, and uses thereof.
211 Citations
22 Claims
-
1. A multispecific antibody comprising
a) a full length antibody consisting of two antibody heavy chains and two antibody light chains wherein the antibody specifically binds to a first antigen; - and
b) one or two single chain Fab fragments that specifically bind to one or two antigens different from the first antigen, wherein each of the single Fab fragments comprises an antibody light chain variable domain (VL) and an antibody heavy chain variable domain (VH) that binds to the antigen that is different from the first antigen, wherein each of the one or two single chain Fab fragments is fused to the full length antibody via a peptide connector at the C- or N-terminus of the heavy chain of the full length antibody. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- and
-
14. A multispecific antibody comprising
a) a full length antibody consisting of two antibody heavy chains and two antibody light chains wherein the antibody specifically binds to a first antigen; - and
b) one to four single chain Fab fragments that specifically bind to one to four antigens different from the first antigen, wherein each of the single chain Fab fragments comprises an antibody light chain variable domain (VL) and an antibody heavy chain variable domain (VH) that binds to the antigen that is different from the first antigen, wherein each of the one to four single chain Fab fragments is fused to the full length antibody via a peptide connector at the C- or N- terminus of the heavy chain of the full length antibody, and wherein each of the one to four single chain Fab fragments comprises a disulfide bond between heavy chain variable domain and light chain variable domain of the single chain Fab fragment. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22)
- and
Specification