Un-supported polymeric film with embedded microbeads

• US 9,436,088 B2
• Filed: 06/12/2007
• Issued: 09/06/2016
• Est. Priority Date: 06/21/2001
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Seul, Michael, Banerjee, Sukanta, Podual, Kairali, Hong, Ye
• Primary Examiner(s): Shibuya, Mark
• Assistant Examiner(s): DO, PENSEE T
• Application Number:
  US11/761,789
• Publication Number:
  US 20070248993A1
• Time in Patent Office: 3,374 Days
• Field of Search: 436/526
• US Class Current: 1/1
• CPC Class Codes: C08J 2333/26 Homopolymers or copolymers of acrylamide or methacrylamide
• CPC Class Codes: C08J 2425/06 Polystyrene
• CPC Class Codes: C08J 3/075 Macromolecular gels
• CPC Class Codes: C08J 3/28 Treatment by wave energy or particle radiation
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G03F 7/0047 characterised by additives for obtaining a metallic or ceramic pattern, e.g. by firing
• CPC Class Codes: G03F 7/164 using electric, electrostatic or magnetic means; powder coating
• CPC Class Codes: Y10T 428/249952 At least one thermosetting synthetic polymeric material layer
• CPC Class Codes: Y10T 428/25 Web or sheet containing structurally defined element or component and including a second component containing structurally defined particles

Abstract

The present invention relates to polymer-bead composites having a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix. The composite may be unattached to a solid support. The encoded beads have different biomolecules attached to their surfaces, and the encoding permits distinguishing beads having different biomolecules attached thereto. The present invention also relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites, including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films.

Claims

1. A polymer-bead composite comprising:

a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix, wherein the composite is not attached to a solid support, and wherein differently-encoded beads have different biomolecules attached to their surfaces and wherein the encoding permits distinguishing of beads having different biomolecules attached thereto from each other.

2. The polymer-bead composite of claim 1 wherein the beads are encoded with a unique chemical or physical characteristic.

3. The polymer-bead composite of claim 2 wherein the encoding is with a fluorescent dye.

4. The polymer-bead composite of claim 1 wherein the biomolecules are peptides, proteins, nucleic acids, oligonucleotide, ligands or receptors.

5. The polymer-bead composite of claim 1 wherein the hydrophilic polymeric matrix is alkylacrylamide or hydroxyacrylalkylate hydrogel.

6. The polymer-bead composite of claim 1 wherein the beads are magnetic beads.

7. The polymer-bead composite of claim 1, wherein the beads are assembled in a hexagonally crystalline configuration.

8. The polymer-bead composite of claim 1, wherein the beads are assembled in a bubble raft arrangement.