Anti-resorptive and bone building dietary supplements and methods of use

• US 9,446,087 B2
• Filed: 10/24/2007
• Issued: 09/20/2016
• Est. Priority Date: 10/24/2006
• Status: Active Grant

• Current Assignee: David J. Fast, David W. Krempin, Haeri Roh-Schmidt, Jatinder Rana, John F. Rebhun, Kevin W. Gellenbeck, Laurie Krempin, Leon M. Wilkins, Mary A. Murray, Silvia R. Da Costa, Yumei Lin
• Original Assignee: Laurie Krempin
• Inventors: Krempin, David W., Murray, Mary A., Lin, Yumei, Gellenbeck, Kevin W., da Costa, Silvia R., Wilkins, Leon M., Roh-Schmidt, Haeri, Rana, Jatinder, Rebhun, John F., Fast, David J.
• Primary Examiner(s): McKelvey, Terry A.
• Assistant Examiner(s): Chen, Catheryne
• Application Number:
  US11/977,696
• Publication Number:
  US 20080199545A1
• Time in Patent Office: 3,254 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A23L 33/105 Plant extracts, their artificial duplicates or their derivatives
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/352 condensed with carbocyclic rings, e.g. methantheline cannabinoids A61K31/658
• CPC Class Codes: A61K 36/185 Magnoliopsida (dicotyledons)
• CPC Class Codes: A61K 36/254 Acanthopanax or Eleutherococcus
• CPC Class Codes: A61K 36/258 Panax (ginseng)
• CPC Class Codes: A61K 36/484 Glycyrrhiza (licorice)
• CPC Class Codes: A61K 36/489 Sophora, e.g. necklacepod or mamani
• CPC Class Codes: A61K 36/804 Rehmannia
• CPC Class Codes: A61K 36/87 Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

Disclosed herein are dietary supplement compositions and methods for increasing or stimulating bone growth, decreasing or preventing bone resorption, increasing bone strength, improving bone structure, and improving bone architecture comprising a first composition comprising a combination of at least two of: quercetin, Rehmannia sp., Rehmannia sp. root, Siberian ginseng, Sophora japonica, licorice, and ipriflavone, wherein the combination of the first composition increases bone morphogenic protein-2 gene, promoter or protein expression; and a second composition comprising an extract of pomegranate in combination with at least one of Siberian ginseng, Ginkgo biloba, green tea, Sophora japonica, Rehmannia sp., grape seed, Dong Quai, and ipriflavone, wherein the combination of the second composition inhibits the expression of RANK-L.

Claims

1. A method of inhibiting bone resorption in a subject comprising administering a combination of from about 500 mg to about 1500 mg of an extract of pomegranate and from about 35 mg to about 200 mg of an extract of grape seed to the subject wherein the pomegranate and grape seed are each present in defined amounts to provide a ratio of pomegranate to grape seed of about 10:

1.

2. The method of claim 1, wherein the administration of the combination results in one or more of the following:

increases bone hardness, increases bone strength, improves bone architecture, and increases trabecular bone volume.

3. A method of inhibiting bone resorption in a subject comprising administering the combination of claim 1 to the subject, wherein the combination inhibits, prevents or decreases the release or expression of RANK-L.

4. The method of claim 1, wherein the combination further comprises ipriflavone.

5. The method of claim 1, wherein the combination comprises approximately 1250 mg of an extract of pomegranate and approximately 125 mg of an extract of grape seed.

6. The method of claim 1, wherein the pomegranate extract comprises at least one punicalagin.

7. The method of claim 4 wherein the ipriflavone is present in an amount from about 400-700 mg.

8. The method of claim 7 wherein the ratio of pomegranate:

grape seed;

ipriflavone;

grape seed extract is about 10;

12;

1.

9. The method of claim 4 wherein the combination further comprises from about 300 to about 700 mg. of green tea.

10. The method of claim 9 wherein the pomegranate, grape seed, ipriflavone, and green tea are each present in defined amounts to provide a ratio of pomegranate:

ipriflavone;

grape seed extract;

green tea of about 10;

10;

1;

1.