Method to isolate, identify, and use embryonic stem cells directed to forebrain interneuron fate

• US 9,487,751 B2
• Filed: 06/02/2010
• Issued: 11/08/2016
• Est. Priority Date: 06/02/2009
• Status: Active Grant

• Current Assignee: Cornell University, Sloan-Kettering Institute For Cancer Research (Memorial Sloan-Kettering Cancer Center)
• Original Assignee: Cornell University, Sloan-Kettering Institute For Cancer Research (Memorial Sloan-Kettering Cancer Center)
• Inventors: Anderson, Stewart A., Maroof, Asif, Studer, Lorenz
• Primary Examiner(s): Marvich, Maria
• Application Number:
  US13/376,039
• Publication Number:
  US 20120148549A1
• Time in Patent Office: 2,351 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/30 Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/08 Antiepileptics; Anticonvulsants
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 25/18 Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
• CPC Class Codes: A61P 25/22 Anxiolytics
• CPC Class Codes: C12N 2500/44 Thiols, e.g. mercaptoethanol
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/15 Transforming growth factor beta (TGF-β)
• CPC Class Codes: C12N 2501/155 Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
• CPC Class Codes: C12N 2501/41 Hedgehog proteins; Cyclopamine (inhibitor)
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2506/45 from artificially induced pluripotent stem cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0619 Neurons

Abstract

The present invention relates to methods of isolating a purified or enriched population of cortical or striatal immature interneuron progenitor cells and the isolated purified or enriched population of immature interneuron progenitor cells. Methods of treating a condition mediated by a loss or deficiency of interneuron function using the purified or enriched population of immature interneuron progenitor cells are also disclosed.

Claims

1. An isolated or a purified preparation of human cortical immature interneuron precursor cells, wherein the human cortical immature interneuron precursor cells of the preparation comprise an exogenous recombinant nucleic acid molecule encoding a marker protein or a therapeutic agent, said recombinant nucleic acid molecule operably linked to at least one of a Lhx6 promoter region and a Lhx6 enhancer region.

2. The isolated or the purified preparation of claim 1, wherein the human cortical immature interneuron precursor cells of the preparation comprise a genomic ally integrated bacterial artificial chromosome.

3. The isolated or the purified preparation of claim 1, wherein the therapeutic agent is selected from the group consisting of neuropeptide Y, galanin, adenosine, and GABA.

4. The isolated or the purified preparation of claim 1, wherein said recombinant nucleic acid molecule is genomically integrated into the cells.