Pre-treatment process for electrochemical amperometric sensor

• US 9,492,118 B1
• Filed: 06/28/2013
• Issued: 11/15/2016
• Est. Priority Date: 06/28/2013
• Status: Active Grant

• Current Assignee: Google LLC (Alphabet Inc.)
• Original Assignee: Life Sciences, LLC
• Inventors: Liu, Zenghe, Pletcher, Nathan
• Primary Examiner(s): JANG, CHRISTIAN YONGKYUN
• Application Number:
  US13/930,861
• Time in Patent Office: 1,236 Days
• US Class Current: 1/1
• CPC Class Codes: A61B 2560/0214 of power generation or supply
• CPC Class Codes: A61B 5/002 Monitoring the patient using a local or closed circuit, e.g. in a room or building A61B5/0017 takes precedence
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1468 using chemical or electrochemical methods, e.g. by polarographic means A61B5/1486 takes precedence
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: A61B 5/6821 Eye

Abstract

A wearable device is provided herein and includes an electrochemical sensor with a working electrode, a reference electrode, and a reagent that selectively reacts with an analyte. In accordance with one embodiment, a process is provided herein and includes applying an electric potential waveform pattern between the working electrode and the reference electrode, the pattern comprising a combination of a constant voltage and at least one voltage waveform including one or more of a triangle waveform, a pulse-step waveform, and a sine waveform, determining a current at the working electrode, the current resulting from the application of the electric potential waveform pattern, determining that the current is less than a threshold level of measured current, and in response, the wearable device stopping the application of the electric potential waveform pattern.

Claims

1. A system comprising:

a wearable device comprising;

an antenna;

an electrochemical sensor that includes a working electrode, a reference electrode, and a reagent that selectively reacts with an analyte; and

a controller electrically connected to the electrochemical sensor and the antenna, wherein the controller is configured to control the electrochemical sensor to obtain a sensor measurement while the wearable device is exposed to a fluid, and use the antenna to transmit the sensor measurement; and

a reader operable in a pre-treatment mode and a measurement mode,

wherein in the pre-treatment mode the reader is configured to (i) wirelessly communicate with the antenna to cause the electrochemical sensor to apply an electric potential waveform pattern between the working electrode and the reference electrode, the pattern comprising a combination of a constant voltage and at least one voltage waveform including one or more of a triangle waveform, a pulse-step waveform, and a sine waveform, (ii) wirelessly communicate with the antenna to receive a pre-treatment measurement obtained while the wearable device is exposed to a buffer solution substantially devoid of the analyte, (iii) determine that the pre-treatment measurement is less than a threshold level, and (iv) in response to the determination, wirelessly communicate with the antenna to cause the wearable device to stop applying the electric potential waveform pattern, and

wherein in the measurement mode the reader is configured to wirelessly communicate with the antenna to receive a tear-film sensor measurement obtained with the wearable device exposed to a tear film and determine a concentration of the analyte in the tear film based on the tear-film sensor measurement.

2. The system of claim 1, wherein the controller includes a potentiostat that is configured to apply the electric potential waveform pattern between the working electrode and the reference electrode.

3. The system of claim 1, wherein the reader is further configured to supply power to the wearable device through the antenna.

4. The system of claim 1, further comprising a user interface configured to receive a selection of one or more of the pre-treatment mode and the measurement mode.

5. The system of claim 1, wherein the threshold level is a threshold percentage of an operating current resulting from the application of electric potential between the working electrode and the reference electrode while the wearable device is exposed to the analyte.

6. The system of claim 1, wherein the reader is configured to determine that the pre-treatment measurement is less than a threshold level by being configured to determine that the pre-treatment measurement has remained within a threshold range for a threshold period of time.

7. The system of claim 1, wherein the reader is further operable in a calibration mode,

wherein in the calibration mode, the reader is configured to (i) wirelessly communicate with the antenna to receive a calibration-solution sensor measurement based on the wearable device being exposed to a calibration solution that contains a concentration of the analyte, (ii) determine a calibration value based on at least the calibration-solution sensor measurement and the concentration of the analyte in the calibration solution, and (iii) store the calibration value in a memory, and

wherein in the measurement mode, the reader is further configured to determine a concentration of the analyte in the tear film based on at least the tear-film sensor measurement and the calibration value.

8. The system of claim 1, wherein the wearable device further comprises a transparent polymeric material having a concave surface and a convex surface, wherein the concave surface is configured to be removably mounted over a corneal surface and the convex surface is configured to be compatible with eyelid motion when the concave surface is so mounted.