Resistance gene and uses thereof

• US 9,512,442 B2
• Filed: 10/28/2008
• Issued: 12/06/2016
• Est. Priority Date: 10/31/2007
• Status: Expired due to Fees

• Current Assignee: The New Zealand Institute For Plant and Food Research Limited
• Original Assignee: The New Zealand Institute For Plant and Food Research Limited
• Inventors: Dare, Andrew Patrick, Rikkerink, Hendrikus Antonius, Hilario-Andrade, Elena Maria, Gardiner, Susan Elizabeth, Yoon, Minsoo, Bus, Vincent Gerardus Maria
• Primary Examiner(s): Kruse, David H
• Application Number:
  US12/740,318
• Publication Number:
  US 20100306875A1
• Time in Patent Office: 2,961 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8282 for fungal resistance

Abstract

The invention provides methods for producing a plant with altered resistance to powdery mildew, the methods comprising transformation of a plant with a genetic construct including a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO: 1 or a variant of fragment thereof. The invention also provides isolated polypeptides, polynucleotides, constructs and vectors useful for producing a plant cell and plants transformed to contain and express the polypeptides, polynucleotides and constructs. The invention also provides plants produced by methods of the invention.

Claims

1. A host cell, plant cell, plant, plant part or plant propagule, that contains a transgene comprising a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:

a) SEQ ID NO;

1,

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6;

wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.

2. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1, wherein the polypeptide comprises the amino acid sequence of at least one of:

a) SEQ ID NO;

1,

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6.

3. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

1.

4. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

5.

5. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1 wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:

6.

6. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1, wherein the polynucleotide comprises the sequence with at least 95% identity to at least one of:

a) SEQ ID NO;

2,

b) SEQ ID NO;

3,

c) SEQ ID NO;

7, and

d) SEQ ID NO;

8.

7. The host cell, plant cell, plant, plant part, or plant propagule, of claim 1, wherein the polynucleotide comprises the sequence of at least one of:

a) SEQ ID NO;

2,

b) SEQ ID NO;

3,

c) SEQ ID NO;

7, and

d) SEQ ID NO;

8.

12. A method for producing a progeny of the plant of claim 1 with increased resistance to powdery mildew, the method comprising at least one of:

a) breeding using the plant as a male or female parent to produce the progeny plant, and

b) asexually reproducing the plant to produce the progeny plant;

wherein the progeny plant contains the transgene.

8. An expression construct comprising a promoter operably linked to a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:

a) SEQ ID NO;

1

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6;

wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant, and wherein the promoter is heterologous to the polynucleotide.

9. A host cell, plant cell, plant, plant part, or plant propagule, comprising the expression construct of claim 8.

10. A method for producing a plant cell or plant with increased resistance to powdery mildew, the method comprising transforming a plant cell or plant with a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 98% identity to the amino acid sequence of at least one of:

a) SEQ ID NO;

1,

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6;

wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.

11. The method of claim 10, wherein the polynucleotide is part of an expression construct.

13. A method for producing a plant cell or plant with increased resistance to powdery mildew, the method comprising transformation of a plant cell or plant comprising a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 95% identity to the amino acid sequence of at least one of:

a) SEQ ID NO;

1,

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6;

wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.

14. A method for producing a polypeptide conferring resistance to powdery mildew, the method comprising expressing in a plant cell or plant a polynucleotide encoding a polypeptide, said polypeptide comprising a sequence with at least 95% identity to the amino acid sequence of at least one of:

a) SEQ ID NO;

1,

b) SEQ ID NO;

5, and

c) SEQ ID NO;

6;

wherein the polypeptide confers resistance to the powdery mildew fungus Podosphaera leucotricha in a Rosaceae plant.