Nucleic-acid extraction method and nucleic-acid extraction cartridge

• US 9,523,091 B2
• Filed: 06/26/2012
• Issued: 12/20/2016
• Est. Priority Date: 07/20/2011
• Status: Active Grant

• Current Assignee: Sony Corporation (Sony Group Corp.)
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Yotoriyama, Tasuku, Nakamura, Tomohiko, Sakamoto, Naohisa, Ito, Kazumine, Abe, Tomoteru, Ohnishi, Michihiro
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US13/533,348
• Publication Number:
  US 20130020201A1
• Time in Patent Office: 1,638 Days
• Field of Search: 536/25.4, 204/450, 204/554, 204/565
• US Class Current: 1/1
• CPC Class Codes: C12N 15/1006 by means of a solid support carrier, e.g. particles, polymers
• CPC Class Codes: C12N 15/101 by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Abstract

The present disclosure provides a nucleic-acid extraction method for carrying out the following procedures in the same cell, the procedures including: performing an ultrasonic process on a sample containing a nucleic acid; adsorbing substances contained in the sample by making use of an adsorption carrier; and condensing the nucleic acid by damming the nucleic acid moving in an electrophoresis phenomenon. In accordance with the nucleic-acid extraction method, it is possible to carry out an ultrasonic process, an adsorption process and an electrophoresis process in the same cell. Thus, it is possible to eliminate an operation to transfer the sample from one cell to another one for each of the processes.

Claims

1. A nucleic-acid extraction method, the method comprising:

inserting a sample containing a nucleic-acid into a negative-electrode side area between a dialysis membrane and a negative electrode;

performing an ultrasonic process on the sample containing a nucleic-acid and adjusting the time and frequency of the ultrasonic process based on the type of the sample;

adsorbing foreign substances contained in the sample using an adsorption carrier after performing the ultrasonic process; and

blocking an electrophoresis movement of the nucleic acid to condense the nucleic-acid on a boundary face of the dialysis membrane after adsorbing the foreign substances, wherein

bead particles are inserted into the negative-electrode side area before the sample containing the nucleic-acid is inserted into the negative-electrode side area.

2. The nucleic-acid extraction method according to claim 1, wherein a cation exchange resin or a zeolite is used as the adsorption carrier.

3. The nucleic-acid extraction method according to claim 2, wherein a strongly acidic cation exchange resin is used as the cation exchange resin.

4. The nucleic-acid extraction method according to claim 1, further comprising blending the bead particles in the sample, and wherein the ultrasonic process is performed after blending the bead particles in the sample.

5. The nucleic-acid extraction method according to claim 1, wherein the foreign substances are each a substance other than the nucleic acid.

6. The nucleic-acid extraction method according to claim 1, further comprising diluting the sample with a buffer solution, wherein the ultrasonic process is performed on the sample after diluting the sample with the buffer solution, and wherein the pH of the buffer solution is between 4.0 and 8.0.

7. The nucleic-acid extraction method according to claim 6, wherein the buffer solution contains a thickening agent.

8. The nucleic-acid extraction method according to claim 7, wherein the thickening agent contains at least one of polyethylene glycol or hydroxy ethyl cellulose.