Double-stranded nucleic acid, use and kit thereof in ribonuclease detection

  • US 9,540,682 B2
  • Filed: 09/10/2012
  • Issued: 01/10/2017
  • Est. Priority Date: 09/20/2011
  • Status: Active Grant
First Claim
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1. A double-stranded nucleic acid which can be cleaved by ribonuclease,wherein the length of the double-stranded nucleic acid is 7-30 base pairs,the base sequence of the first single strand of the double-stranded nucleic acid contains at least one CA base sequence or UA base sequence, the base sequence of the second single strand of the double-stranded nucleic acid contains UG base sequence or UA base sequence which is complementary to the CA base sequence or the UA base sequence in the first single strand,one end of the double-stranded nucleic acid is attached to an fluorescence donor group, the other end the double-stranded nucleic acid is attached to an fluorescence acceptor group, and energy transfer can occur between the fluorescence donor group and the fluorescence acceptor group.

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