Double-stranded nucleic acid, use and kit thereof in ribonuclease detection
First Claim
1. A double-stranded nucleic acid which can be cleaved by ribonuclease,wherein the length of the double-stranded nucleic acid is 7-30 base pairs,the base sequence of the first single strand of the double-stranded nucleic acid contains at least one CA base sequence or UA base sequence, the base sequence of the second single strand of the double-stranded nucleic acid contains UG base sequence or UA base sequence which is complementary to the CA base sequence or the UA base sequence in the first single strand,one end of the double-stranded nucleic acid is attached to an fluorescence donor group, the other end the double-stranded nucleic acid is attached to an fluorescence acceptor group, and energy transfer can occur between the fluorescence donor group and the fluorescence acceptor group.
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Accused Products
Abstract
Provided are a double-stranded nucleic acid and use thereof in ribonuclease detection, and a ribonuclease detection method and use thereof in tumor detection and/or diagnosis. Specifically, a double-stranded nucleic acid substrate comprises at least one ribonuclease sensitive site. The activity and content of the ribonuclease in a sample are detected by analyzing the degradation of the double-stranded nucleic acid substrate by the ribonuclease. Also provided are a ribonuclease detection kit and a tumor detection kit.
4 Citations
22 Claims
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1. A double-stranded nucleic acid which can be cleaved by ribonuclease,
wherein the length of the double-stranded nucleic acid is 7-30 base pairs, the base sequence of the first single strand of the double-stranded nucleic acid contains at least one CA base sequence or UA base sequence, the base sequence of the second single strand of the double-stranded nucleic acid contains UG base sequence or UA base sequence which is complementary to the CA base sequence or the UA base sequence in the first single strand, one end of the double-stranded nucleic acid is attached to an fluorescence donor group, the other end the double-stranded nucleic acid is attached to an fluorescence acceptor group, and energy transfer can occur between the fluorescence donor group and the fluorescence acceptor group.
Specification