Fungal endophytes for improved crop yields and protection from pests

• US 9,545,111 B2
• Filed: 12/09/2015
• Issued: 01/17/2017
• Est. Priority Date: 11/06/2013
• Status: Active Grant

• Current Assignee: Texas A&M University System
• Original Assignee: Texas A&M University System
• Inventors: Sword, Gregory A.
• Primary Examiner(s): Pryor, Alton
• Application Number:
  US14/964,440
• Publication Number:
  US 20160192662A1
• Time in Patent Office: 405 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom

Abstract

The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

Claims

1. A method for improving a trait in a cotton plant, the method comprising:

contacting a cotton a seed of said cotton plant with a formulation comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are Dothideomycetes capable of producing substances that are beneficial to plants or detrimental to pests or both, and are present in the formulation in an amount effective to decrease the colonization frequencies of endophytes of genus Alternaria that are native to the cotton plant and to provide a benefit to the cotton plant compared to a reference cotton plant grown from a seed untreated with the Dothideomycetes facultative fungal endophytes, wherein the benefit is selected from the group consisting of increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, enhanced resistance to drought stress and increased yield.

2. The method of claim 1, wherein the increased square retention is measured in first fruiting position on branches as flowers begin to develop.

3. The method of claim 1, wherein the increased biomass is measured at mid-season in a field.

4. The method of claim 1, wherein the enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt or time to death as compared to a seedling grown from a reference seed.

5. The method of claim 1, wherein yield is increased by at least about 2%.

6. The method of claim 1, wherein the formulation contains at least 100 (10^2) spores/ml or 100,000 (10^5) spores/g dry weight of the facultative fungal endophytes.

7. The method of claim 1, wherein the benefit is increased square retention.

8. The method of claim 1, wherein the benefit is increased boll retention.

9. The method of claim 1, wherein the benefit is increased biomass.

10. The method of claim 1, wherein the benefit is increased root length.

11. The method of claim 1, wherein the benefit is increased root mass.

12. The method of claim 1, wherein the benefit is enhanced resistance to drought stress.

13. The method of claim 1, wherein the benefit is increased yield.

14. A synthetic combination of a cotton seed and purified filamentous, spore-forming facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are Dothideomycetes capable of producing substances that are beneficial to plants or detrimental to pests or both and are present in an amount effective to decrease the colonization frequencies of endophytes of genus Alternaria that are native to the cotton plant grown from the seed and to provide a benefit to the cotton plant grown from the seed compared to a reference cotton plant grown from a seed untreated with the Dothideomycetes facultative fungal endophytes, wherein the benefit is selected from the group consisting of increased square retention, increased boll retention, increased biomass, increased root length, increased root mass, enhanced resistance to drought stress and increased yield.

15. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present in an amount effective to provide the benefit of increased yield by about 2%.

16. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 100 (10^2) spores/seed on the surface of the seed.

17. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 1,000 (10^3) spores/seed on the surface of the seed.

18. The synthetic combination of claim 14, wherein the facultative fungal endophytes are present at a concentration of at least 10,000 (10^4) spores/seed on the surface of the seed.

19. The synthetic combination of claim 14, wherein the facultative fungal endophytes are in spore form.

20. The synthetic combination of claim 14, comprising at least 2 species of facultative endophytes.

21. The synthetic combination of claim 14, wherein the facultative fungal endophyte is native to the cotton plant grown from the seed.