Irreversible electroporation to create tissue scaffolds
First Claim
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1. A method of making an engineered tissue by electrical ablation comprising:
- positioning at least one electrode in a target region containing tissue cells;
applying a plurality of electrical pulses through the electrode in an amount sufficient to induce cell death by electrical ablation of the tissue cells in the target region in vivo to create an engineered tissue scaffold, wherein the step of applying leaves intact vascular structures present in the target region; and
seeding the ablated target region in vivo or in vitro and allowing seeded cells to proliferate to create the engineered tissue.
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Abstract
The present invention provides engineered tissue scaffolds, engineered tissues, and methods of using them. The scaffolds and tissues are derived from natural tissues and are created using non-thermal irreversible electroporation (IRE). Use of IRE allows for ablation of cells of the tissue to be treated, but allows vascular and neural structures to remain essentially unharmed. Use of IRE thus permits preparation of thick tissue scaffolds and tissues due to the presence of vasculature within the scaffolds. The engineered tissues can be used in methods of treating subjects, such as those in need of tissue replacement or augmentation.
809 Citations
41 Claims
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1. A method of making an engineered tissue by electrical ablation comprising:
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positioning at least one electrode in a target region containing tissue cells; applying a plurality of electrical pulses through the electrode in an amount sufficient to induce cell death by electrical ablation of the tissue cells in the target region in vivo to create an engineered tissue scaffold, wherein the step of applying leaves intact vascular structures present in the target region; and seeding the ablated target region in vivo or in vitro and allowing seeded cells to proliferate to create the engineered tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of making an engineered tissue by irreversible electroporation comprising:
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positioning at least one electrode in a target region containing tissue cells; applying a plurality of electrical pulses through the electrode in an amount sufficient to induce cell death by irreversible electroporation of the tissue cells in the target region in vivo, to create an engineered tissue scaffold; wherein the step of applying leaves intact vascular structures present in the target region; and seeding the engineered tissue scaffold in vivo or in vitro and allowing seeded cells to proliferate to create the engineered tissue. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
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27. A method of making an engineered tissue scaffold by electrical ablation comprising:
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positioning at least one electrode in a target region containing tissue cells; applying a plurality of electrical pulses through the electrode in an amount sufficient to induce cell death by electrical ablation of the tissue cells in the target region; wherein the step of applying leaves intact vascular structures present in the target region in vivo; and removing the ablated tissue region from a body. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 40, 41)
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35. A method of making an engineered tissue by electrical ablation comprising:
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positioning at least one electrode in a target region containing tissue cells; applying, in vivo, a plurality of electrical pulses through the electrode in an amount sufficient to induce cell death by electrical ablation of the tissue cells in the target region to create an engineered tissue scaffold, wherein the step of applying is based on a protocol that leaves intact vascular structures for microcirculation in the target region; seeding the ablated target region and allowing seeded cells to proliferate to create the engineered tissue. - View Dependent Claims (36, 37, 38, 39)
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Specification