Endophytic microbial symbionts in plant prenatal care

• US 9,687,001 B2
• Filed: 03/07/2016
• Issued: 06/27/2017
• Est. Priority Date: 02/05/2013
• Status: Active Grant

• Current Assignee: University Of Saskatchewan
• Original Assignee: University Of Saskatchewan
• Inventors: Vujanovic, Vladimir, Germida, James J.
• Primary Examiner(s): Kruse, David H
• Application Number:
  US15/063,350
• Publication Number:
  US 20160174570A1
• Time in Patent Office: 477 Days
• Field of Search: None
• CPC Class Codes: A01H 17/00 Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza lichens A01H15/00
• CPC Class Codes: A01H 3/00 Processes for modifying phenotypes , e.g. symbiosis with bacteria A01H4/00 takes precedence
• CPC Class Codes: A01N 25/00 Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application , e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• CPC Class Codes: A01N 63/00 Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03
• CPC Class Codes: A01N 63/28 Streptomyces
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: B09C 1/105 using fungi or plants
• CPC Class Codes: B09C 2101/00 In situ
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/465 Streptomyces
• CPC Class Codes: C12R 2001/645 Fungi ; Processes using fungi
• CPC Class Codes: C12R 2001/80 Penicillium

Abstract

The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom.

Claims

1. A method of increasing resistance to nitrogen stress in at least one agricultural crop, said method comprising mechanically or manually inoculating the seed of the agricultural crop with a composition comprising fungal endophytes capable of causing populations of seeds from at least two different crops to have increased yield under nitrogen stress conditions compared to control seeds, wherein the fungal endophytes are of genus Sarocladium and are deposited under IDAC 200312-05.

2. The method of claim 1, wherein the agricultural crop is selected from the group consisting of cereal, pulse, and canola.

3. The method of claim 2, wherein the crop is a cereal.

4. The method of claim 3, wherein the crop is wheat.