Method and fuel cell for electrochemical measurement of analyte concentration in vivo

• US 9,709,520 B2
• Filed: 11/23/2015
• Issued: 07/18/2017
• Est. Priority Date: 03/11/2010
• Status: Active Grant

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Inventors: Ocvirk, Gregor, Klker, Karl-Heinz
• Primary Examiner(s): WEARE, MEREDITH H
• Application Number:
  US14/948,837
• Publication Number:
  US 20160077036A1
• Time in Patent Office: 603 Days
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 60/50 Fuel cells

Abstract

The invention relates to a method for the electrochemical measurement of an analyte concentration in vivo, comprising a fuel cell with which the analyte to be measured is reacted catalytically with an enzyme contained in an enzyme layer and which supplies an electrical voltage, dependent on the analyte concentration to be measured, between an anode and a cathode, which voltage is measured. In the catalytic reaction of the analyte to be measured in the enzyme layer, a product is generated which, as fuel of the fuel cell, oxidizes on the anode and is reduced on the cathode. The invention further relates to a fuel cell for such a method.

Claims

1. A method for electrochemical measurement of an analyte concentration in vivo, comprising:

providing a fuel cell comprising an enzyme layer, an anode and a cathode;

at least partially implanting the fuel cell in a user so that a bodily fluid having the analyte contacts the enzyme layer;

catalytically converting the analyte to be measured with an enzyme contained in the enzyme layer, wherein the catalytic conversion generates a fuel for the fuel cell which is oxidized on the anode and is reduced on the cathode;

generating an electrical voltage between the anode and the cathode, said voltage corresponding to the concentration of the analyte; and

determining the analyte concentration as a function of the electrical voltage.

2. The method of claim 1, wherein the oxidation of the fuel is supported by a catalyst.

3. The method of claim 2, wherein the catalyst comprises a metal oxide or a metallo-organic compound.

4. The method of claim 1, wherein the anode and the cathode are connected to each other via an electrical resistor, the method further comprising measuring the electrical voltage between the anode and the cathode as a voltage drop at the resistor.

5. A method for electrochemical measurement of an analyte concentration in vivo, comprising:

providing a fuel cell comprising an enzyme layer, an anode and a cathode, the cathode having a surface made of a different material than that of the anode;

at least partially implanting the fuel cell in a user so that a bodily fluid having the analyte contacts the enzyme layer;

catalytically converting the analyte to be measured with an enzyme contained in the enzyme layer, wherein the catalytic conversion generates a fuel for the fuel cell;

oxidizing the fuel on the anode and reducing the fuel on the cathode to thereby produce a signal that corresponds to analyte concentration; and

measuring the signal to determine the analyte concentration.