Production of proteins in glutamine-free cell culture media

CAFC
  • US 9,714,293 B2
  • Filed: 03/26/2015
  • Issued: 07/25/2017
  • Est. Priority Date: 08/11/2009
  • Status: Active Grant
First Claim
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1. A process for producing a polypeptide in a host cell expressing said polypeptide, comprising culturing the host cell in a production phase of the culture in a glutamine-free production culture medium containing asparagine and aspartic acid, wherein the asparagine is added at a concentration in the range of 7.5 mM to 15 mM and wherein the aspartic acid is added at a concentration in the range of 1 mM to 10 mM.

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