Spectrometric systems and methods for improved focus localization of time- and space-varying measurements

• US 9,739,663 B2
• Filed: 04/29/2013
• Issued: 08/22/2017
• Est. Priority Date: 04/30/2012
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Haider, Clifton R., Gilbert, Barry K., Delp, Gary S., Rose, James A.
• Primary Examiner(s): Geisel, Kara E
• Assistant Examiner(s): Ayub, Hina F
• Application Number:
  US14/397,814
• Publication Number:
  US 20150109617A1
• Time in Patent Office: 1,576 Days
• Field of Search: 356300
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14552 Details of sensors specially adapted therefor
• CPC Class Codes: G01J 3/0237 Adjustable, e.g. focussing
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3

Abstract

A system and method of dynamically localizing a measurement of parameter characterizing tissue sample with waves produced by spectrometric system at multiple wavelengths and detected at a fixed location of the detector of the system. The parameter is calculated based on impulse response of the sample, reference data representing characteristics of material components of the sample, and path lengths through the sample corresponding to different wavelengths. Dynamic localization is effectuated by considering different portions of a curve representing the determined parameter, and provides for the formation of a spatial map of distribution of the parameter across the sample. Additional measurement of impulse response at multiple detectors facilitates determination of change of the measured parameter across the sample as a function of time.

Claims

1. A method for determining a characteristic of a biological sample with a spectrometric system, the spectrometric system including at least one transmitter and at least one receiver, the method comprising:

with a data-processing unit of the system, acquiring an output from the at least one receiver caused by the at least one transmitter'"'"'s emission of at least one electromagnetic wave (EMW) at at least one corresponding wavelength, the at least one transmitter and the at least one receiver being operably juxtaposed with the sample, the output representing an optical property of the sample determined along a path from the at least one transmitter to the at least one receiver through the sample, said path defined by a gradient of concentration of a material component in the sample;

receiving, with the data-processing unit of the system, reference data representing an empirically-defined spectrally-dependent characteristic of the sample;

determining an impulse response of the sample, the impulse response being associated with emission of the at least one EMW by the at least one transmitter; and

determining, as a function of time, at least one wavelength-dependent material parameter characterizing the sample based on a determined impulse response and the reference data.

2. A method according to claim 1, wherein the acquiring an output includes acquiring an output representing the optical property including at least one of wavelength-dependent scattering, absorption, and anisotropy of the sample, said optical property having been determined without the use of an arterial pulse in the sample.

3. A method according to claim 1, wherein the determining, as a function of time, a wavelength-dependent material parameter characterizing the sample includes determining a wavelength-dependent material parameter characterizing the sample based on an averaged wavelength-dependent path length that has been estimated with the use of the determined impulse response and scattering in the sample, said average wavelength dependent path being different from a straight path between the at least one optical transmitter and the at least one receiver.

4. A method according to claim 3, further comprising:

sampling a curve representing the at least one wavelength-dependent material parameter as a function of time to obtain values of said at least one wavelength-dependent material parameter at sampled points in time, and

forming a weighting function by calculating variance values among the values of said at least one wavelength-dependent material parameter at the sampled points in time, the weighting function being defined by said variance values as a function of time.

5. A method according to claim 4, further comprising:

modifying the at least one wavelength-dependent material parameter characterizing the sample based on (i) the computed impulse response that has been weighted by the weighting function and (ii) the reference data.

6. A method according to claim 1, wherein said determining the at least one wavelength-dependent material parameter includes determining one or more of a level of oxygenation of the sample and a concentration of a chosen species in the sample,

and further comprising:

at different wavelengths, modulating respectively-corresponding EMWs with first and second excitation sequences produced at the at least one transmitter, the first and second excitation sequences being orthogonal to one another.

7. A method according to claim 1, further comprising deriving a variation of a cardiac stroke volume based on said at least one wavelength-dependent material parameter characterizing the sample.

8. A method according to claim 1, wherein the determining the impulse response includes determining the impulse response of the sample based on the output, the output being a time-dependent characteristic of a pair formed by the at least one transmitter and the at least one receiver, the pair identified at the data-processing unit of the system by a respectively corresponding excitation sequence series of pulses produced by the at least one transmitter, a first excitation sequence series of pulses at a first wavelength being orthogonal to a second excitation sequence series of pulses at a second wavelength.

9. A method according to claim 1, further comprising forming a spatial map of the at least one wavelength-dependent material parameter across a cross-section of the sample located between the at least one transmitter and the at least one receiver based on different portions of a curve representing said at least one wavelength-dependent material parameter as a function of time.

10. A method according to claim 1, further comprising adding a chemical substance to the biological sample, and wherein said determining the at least one wavelength-dependent material parameter includes determining a change in the at least one wavelength-dependent material parameter caused by said adding.

11. A method according to claim 10, further comprising generating said emission by the at least one transmitter as electromagnetic pulses in a defined excitation sequence and transmitting said pulses through a delay line with multiple delay taps to (i) enhance said determining the change in the at least one wavelength-dependent material parameter and (ii) reduce a cost of the determining of said impulse response.

12. A method according to claim 1, further comprising repositioning at least one of one or more transmitters and one or more receivers along a surface of the biological sample to enhance localization of said determining the at least one wavelength-dependent material parameter based on an image of the biological sample created as a result of said repositioning, the image representing a structure of the biological sample.

13. A method according to claim 1, wherein the sample is homogeneous.