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Differentiation of human embryonic stem cells

  • US 9,744,195 B2
  • Filed: 06/26/2015
  • Issued: 08/29/2017
  • Est. Priority Date: 07/31/2007
  • Status: Active Grant
First Claim
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1. A method for increasing insulin in a patient having Type 1 diabetes comprising:

  • and differentiating pancreatic endoderm cells into a population of pancreatic endocrine cells by culturing pancreatic endoderm cells in medium supplemented with glucose at a concentration from about 10 mM to about 20 mM and treating with a factor selected from the group consisting of;

    a gamma secretase inhibitor, Exendin-4, and a combination of Exendin-4 and hepatocyte growth factor; and

    providing the population of pancreatic endocrine cells to the patient, wherein the cells are provided by implanting encapsulated cells into the liver or by infusing the cells into the hepatic portal vein, wherein cells in the population of pancreatic endocrine cells express insulin.

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